产胞外多糖植物内生菌的分离和鉴定  

Isolation and Characterization of Endophytic Strains Producing Exopolysaccharide

作　　者：黄怡诚 刘旸[1] 庞昕[1] 马中瑞[1] 韩东雷 陈敏[1] 

机构地区：[1]山东大学生命科学院微生物技术国家重点实验室,济南250100

出　　处：《生物技术通报》2014年第4期147-151,共5页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31270983;31070824);教育部留学回国人员科研启动基金资助项目第45批;山东省自然科学基金(2009ZRB019SQ)

摘　　要：利用平板涂布法从胡萝卜、芦荟、土豆、地瓜和紫薯样品中分离得到24株细菌,利用苯酚硫酸法测定菌株胞外多糖的产量,最终得到3株高产胞外多糖的细菌菌株25-Z-1、25-Z-3和12-L-6。经16S rDNA序列测定及系统发育分析,从紫薯样品中分离得到的25-Z-1与Bacillus pumilus strain ST277处于同一分支,相似性达到100%;25-Z-3与Bacillus cereus strain Se05处于同一分支,相似性达到99%;从芦荟中分离得到的12-L-6与Bacillus sp.GZT处于同一分支,相似性为99%。在LB液体培养基中,发酵温度30℃,摇床转数170 r/min条件下,发酵7 d后胞外多糖产量可分别达30.3 mg/L、29.1 mg/L及28.2 mg/L。In this study, 24 strains were isolated from carrot, aloe, potato, sweet potato and purple potato. As screening by phenol-sulfuric acid assay, three strains, 25-Z-1, 25-Z-3 and 12-L-6, were proved to be as high exopolysaccharide producing strains. The results of 16S rDNA sequence determination showed that 25-Z-1 and 25-Z-3 isolated from purple potato was about 100%identity compared with Bacillus pumilus strain ST277 and 99%identity compared with Bacillus cereus strain Se05, respectively. Meanwhile the 12-L-6 isolated from aloe identified as Bacillus sp. GZT, with the similarity is of 99%. Culture in LB medium, 30℃and 170 r/min for 7 d, yield of EPS could reach to 30.3 mg/L, 29.1 mg/L and 28.2 mg/L, respectively.

关 键 词：植物内生菌 胞外多糖 16S RDNA 

分 类 号：Q93[生物学—微生物学]