柴胡皂甙D通过非mTOR依赖途径诱导HeLa细胞自噬  

Study on saikosaponin D to induce autophagy in HeLa Cells via a Mtor- independent pathway

作　　者：袁鹏[1] 金琨琪[1] 程威[1] 杨志强[1] 施晓晨 史祺云[1] 申凤鸽[1] 于录[1] 

机构地区：[1]人兽共患病研究教育部重点实验室吉林大学人兽共患病研究所,长春130062

出　　处：《黑龙江畜牧兽医》2014年第5期27-29,227,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31172364;31000822;3127 1951);国家"十二五"重大传染病专项课题(2012ZX1000300 2);深圳市重点实验室建设扶持计划项目(SW201110026);深圳市药物研发中相关化合物测试与解析高端服务公共技术平台项目(深发改2011-705);深圳市战略新兴产业发展专项资金知识创新计划项目(JCYJ20130401173241464);深圳市基础研究计划项目(JCYJ20130401172016183;YJ20120616142424 467);深圳市基础研究实验室提升计划项目(ZDSY201206161 41302982)

摘　　要：为了研究柴胡皂甙D能否诱导HeLa细胞发生自噬,并初步探讨其机制,试验采用激光共聚焦显微镜、免疫印记等技术评价经柴胡皂甙D处理后的HeLa细胞的自噬水平。结果表明:经柴胡皂甙D处理后,激光共聚焦显微镜观察到GFP-LC3斑点显著增加;柴胡皂甙D能显著增加LC3-Ⅱ的表达;但自噬关键蛋白mTOR没有变化。说明柴胡皂甙D能诱导HeLa细胞发生自噬,并通过非mTOR依赖途径。To study whether saikosaponin D can or can not induce autophagy of HeLa cells, and to initially investigate its mechanisms. The con- focal laser scanning microscopy （CLSM） , western - blot, and other techniques were used to evaluate the levels of autophagy of HeLa ceils after treatment with saikosaponin D. The results showed a significant increase was observed in the number of GFP - LC3 puncta using CLSM after treatment with saikosaponin D ; saikosaponin D could significantly increase the expression of LC3 - ]I, but the levels of mTOR for key autoph- agic proteins were unchanged. The results indicate that saikosaponin D can induce autophagy in HeLa cells via a roTOR - independent pathway.

关 键 词：柴胡皂甙D 自噬 HELA细胞 LC3 MTOR 

分 类 号：S853.72[农业科学—临床兽医学] Q254[农业科学—兽医学]