玉米秸秆纤维素降解菌的分离纯化与产酶工艺优化  

Screening of a Corn Straw Cellulose-degrading Strains and Optimization of the Cellulase Producing Conditions

作　　者：向殿军[1] 满丽莉[1] 张春凤[1] 张彩玲[1] 张娣[1] 殷奎德[2] 曹天旭[1] 王丽娜[3] 

机构地区：[1]黑龙江农业经济职业学院,黑龙江牡丹江157041 [2]黑龙江八一农垦大学生命科学技术学院,黑龙江大庆163319 [3]黑龙江省农业科学院大庆分院,黑龙江大庆163319

出　　处：《农产品加工（创新版）（中）》2014年第4期37-39,43,共4页Farm Products Processing

基　　金：牡丹江市科学技术计划项目"玉米秸秆纤维素降解菌的选育及应用研究"(G2013n0012)的阶段性研究成果

摘　　要：从富含腐烂玉米秸秆的土壤中分离筛选到1株能降解纤维素的细菌K01,经形态观察和16S rDNA序列分析,初步鉴定该菌属于美洲爱文氏菌。对该菌产酶条件进行优化,结果表明在接种种龄24 h,培养液初始pH值7,接种量3%,培养时间48 h的优化条件下,酶活力达1.58 IU/mL。该菌株对玉米秸秆降解效果较好,优化条件下7 d降解率达56.1%。A cellulose-decomposing fungus strain is isolated from soil containing rich rotten corn straw. Based on morphology and 16St DNA sequence analysis, it is initially classified into the genus of Penicillium. And the optimum conditions for CMCase production are determined as follows： seed age of 24 hours, initial pH value of medium of 7, inoculum amour of 3%, cnlmre time of 48 hours. Under the optimal conditions, the CMCase activity reaches 1.58 IU/mL. The strain K01 effectively degrades the corn straw, and the degradation rate reaches 56.1% in seven days.

关 键 词：玉米秸秆 纤维素降解菌 16S RRNA 产酶条件 

分 类 号：S182[农业科学—农业基础科学]