蜡蚧霉的固体发酵动力学研究  

Kinetics of the Solid-state Fermentation of Lecanicillium Lecanii

作　　者：张莹玲 楚占龙 金凡[1] 张玲玉[1] 薛皎亮[1] 谢映平[1] 

机构地区：[1]山西大学生命科学学院,山西太原030006

出　　处：《山西大学学报（自然科学版）》2014年第2期293-298,共6页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(31070584);国家公益性行业(农业)科研专项(200803006);2013山西省高等学校大学生创新创业训练项目

摘　　要：研究蜡蚧霉Lecanicillium lecanii(Zimmermann.)V3.4505菌株固体发酵动力学,为规模化生产提供相关指标的动态监测和各批次之间质量控制的依据。采用稻谷壳、麦麸、玉米粉配制固体培养基,培养过程中每隔36h取样,测定菌体的产孢量,底物的总糖、总氮的变化,采用Logistic方程构建菌株发酵过程中菌体生长和底物消耗的动力学模型。发现菌株生长经历缓慢生长期、对数生长期、稳定生长期3个阶段。培养基中总糖和总氮的消耗与菌体生长曲线的3个阶段相对应,在菌体缓慢生长期,总糖和总氮的消耗也较少,进入对数生长期,菌体生长对底物的消耗量急剧加大,从7.5d后进入稳定期,底物消耗的速度也变缓;对实验数据进行处理,得到了菌株发酵过程的动力学数学模型和模型参数,总糖和总氮消耗模型的拟合度为0.834 2和0.744 6。In order to provide a reference for the dynamic monitoring of related factors and quality control of each batch for large-scale production of Lecanicillium lecanii （Zimmermann.）. The solid-state fermentation kinetics of the V3. 4505 strain was studied. Rice hull,wheat bran and cornmeal were used to prepare the solid medium for the strain culturing. The samples were collected every 36 h intervals during the fermentation cycle. The spore production of the strain, and changes of total sugar and total nitrogen in the solid medium were determined. The kinetic models of strain growth and substrate consumption were established based on the Logistic equation. It was found that strain growth experienced three stages, the slow growth, logarithmic growth, and stable growth. The consumption of total sugar and total nitrogen in the solid medium responded to the three stages of the fungal growth. As the strain slowly growing,its consumptions for total sugar and total nitrogen were much less, but within the logarithmic growth phase of fungus, the substrate consumption increased sharply, and with the fungus growth entering the stable period at 7.5 d,substrate consumption rate also slowed down. The experimental data were processed by using the Origin 7.5 software. The kinetic models and the model parameters were established for indicating the fungal solid state fermentation process. The fitting degrees of the models with total sugar and total nitrogen were 0. 834 2 and 0. 744 6,respectively.

关 键 词：昆虫病原真菌 蜡蚧霉 固体发酵 动力学 

分 类 号：Q965[生物学—昆虫学]