重组血管内皮受体F3肽-铜绿假单胞菌外毒素A蛋白抑制肿瘤生长的实验研究  

Recombinant Vascular Endothelial Receptor F3 Peptide-PE39KDEL Protein Inhibit Tumor Growth

作　　者：郭凯[1] 陈泉[1] 周红姿[1] 陈凯[1] 李纪顺[1] 扈进冬[1] 杨合同[1] 

机构地区：[1]山东省科学院中日友好生物技术研究中心,山东省应用微生物重点实验室,济南250014

出　　处：《科学技术与工程》2014年第11期127-130,共4页Science Technology and Engineering

基　　金：国家"重大新药创制"科技重大专项(2009ZX09102-214);山东省科技发展计划(2012GSF12114);山东省科学院博士基金(201207-18)资助

摘　　要：检测融合表达的F3肽-铜绿假单胞菌外毒素A(F3-PE39KDEL)在抗肿瘤细胞及活体肿瘤抑制方面的作用效果。用MTT法检测F3-PE39KDEL对肿瘤细胞株的抑制活性。小鼠半致死剂量LD50检测毒理学性质,裸鼠成瘤实验检测F3-PE39KDEL对荷瘤裸鼠中肿瘤的生长抑制。结果表明,融合蛋白F3-PE39KDEL能很好抑制肿瘤细胞和人结肠癌的生长,同时具有较低的生物学毒性。说明F3-PE39KDEL具有抑制人结肠癌生长的潜在应用价值。Investigate the suppression effect of F3-PE39KDEL in tumor cells and in vivo tumor growth, activities of F3-PE39KDEL inhibiting tumor cells were detected by MTT method, and the toxicology was determined by mice Lethal Dose test. Balb/c nude mice were used as model to detect the activity of F3-PE39KDEL on inhibiting tumor growth. Results show that F3-PE39KDEL can inhibit tumor SW620 growth in vivo with low biological toxicity. It is F3-PE39KDEL has a potent utility in inhibiting tumor SW620 growth.

关 键 词：F3肽 铜绿假单胞菌外毒素A 受体 肿瘤 

分 类 号：R735.55[医药卫生—肿瘤]