Effect of acupotomy on nitric oxide synthase and beta-endorphin in third lumbar vertebrae transverse process syndrome model rats  

作　　者：Changqing Guo Naigang Liu Xiaohong Li Hongmei Sun Bo Hu Jing Lu Yan Guo Chuxi Liang Hong Xu Haixia Wu 

机构地区：[1]School of Acupuncture & Moxibustion and Tuina,Beijing University of Chinese Medicine [2]Department of Acupuncture & Moxibustion,China-Japan Friendship Hospital [3]School of Basic Medical Sciences,Beijing University of Chinese Medicine [4]Department of Acupuncture & Moxibustion and Physiotherapy,Beijing Shuili Hospital

出　　处：《Journal of Traditional Chinese Medicine》2014年第2期194-198,共5页中医杂志（英文版）

基　　金：Supported by a Grant from the National Basic Research Program of China (973 Program)(No.2006CB504508)

摘　　要：OBJECTIVE： To explore the long-term effects and pain relief mechanism of acupotomy by observing changes in nitric oxide synthase （NOS） and beta-en- dorphin （~3-EP） in the hypothalamus, spinal cord, and peripheral blood of rats with third lumbar ver- tebrae （L3） transverse process syndrome. METHODS： Twenty-eight SD rats were randomly as- signed to normal, model, electroacupuncture （EA）, and acupotomy group. The last three groups were put through an operation to emulate L3 transverse process syndrome. Fourteen days after the simulation operation, EA and acupotomy treatments were applied to the respective groups. Fifty-six days afterthe simulation operation, biochemistry tests and enzyme-linked immunosorbent assay were used to measure NOS and 13-EP in the hypothalamus, spinal cord, and peripheral blood. RESULTS： Rats with the simulation operation showed significantly higher levels of NOS and II3-EP in the hypothalamus, spinal cord, and peripheral blood than those in the normal group. The EA and acupotomy groups had significantly lower levels of NOS and β-EP than those in the model group. There was no statistical difference between the EA and acupotomy groups. CONCLUSION： EA and acupotomy treatments significantly lowered NOS and β-EP levels in the hypothalamus, spinal cord, and peripheral blood and alleviated L3 transverse process syndrome.OBJECTIVE: To explore the long-term effects and pain relief mechanism of acupotomy by observing changes in nitric oxide synthase(NOS) and beta-endorphin(β-EP) in the hypothalamus, spinal cord,and peripheral blood of rats with third lumbar vertebrae(L3) transverse process syndrome.METHODS:Twenty-eight SD rats were randomly assigned to normal, model, electroacupuncture(EA),and acupotomy group. The last three groups were put through an operation to emulate L3transverse process syndrome. Fourteen days after the simulation operation, EA and acupotomy treatments were applied to the respective groups. Fifty-six days afterthe simulation operation, biochemistry tests and enzyme-linked immunosorbent assay were used to measure NOS and β-EP in the hypothalamus, spinal cord, and peripheral blood.RESULTS: Rats with the simulation operation showed significantly higher levels of NOS and β-EP in the hypothalamus, spinal cord, and peripheral blood than those in the normal group. The EA and acupotomy groups had significantly lower levels of NOS and β-EP than those in the model group.There was no statistical difference between the EA and acupotomy groups.CONCLUSION: EA and acupotomy treatments significantly lowered NOS and β-EP levels in the hypothalamus, spinal cord, and peripheral blood and alleviated L3transverse process syndrome.

关 键 词：ACUPOTOMY Small needle knife ELECTROACUPUNCTURE L3 transverse process syndrome Nitric oxide synthase BETA-ENDORPHIN 

分 类 号：R245[医药卫生—针灸推拿学]