双适配体夹心微阵列芯片在凝血酶检测中的应用  

A Sandwich Aptamer Microarray Chip for Thrombin Detection

作　　者：满燕[1] 吕雪飞[1] 彭广[1] 宋达[1] 庆宏[1] 

机构地区：[1]北京理工大学生命学院,北京100081

出　　处：《生命科学仪器》2014年第1期69-71,共3页Life Science Instruments

基　　金：国家自然科学基金项目(21275019)资助

摘　　要：核酸适配体不仅具备抗体分子的专一性识别性能,而且还具备其独特的优异特性。凡涉及到抗体的识别范畴,几乎都可以用适配体来取代抗体。微阵列芯片具有高通量、微量化和自动化等优点。本论文以凝血酶为检测对象,在优化氨基化适配体(T29)在醛基化玻璃基片上的固定条件,并对固定结果进行表征的基础上,设计制作了用于凝血酶检测的基于双适配体夹心原理的微阵列芯片。结果表明,氨基化适配体T29-1在醛基化玻璃基片上的固定浓度为5μM,最佳的固定缓冲液为含有37.5%DMSO的3×SSC,在微阵列芯片加工制作的基础上,利用双适配体夹心法的原理初步实现了凝血酶的检测,这为利用微阵列芯片实现多种物质的同时检测奠定了方法学基础。Aptamers not only have the specific recognition ability of antibody molecules, also have unique merits. Where it comes to identifying areas of antibodies, aptamers can be used almost to replace antibodies. Meanwhile microarray chip has the advantages in high flux, trace amount and automation. In this study, a microarray chip was designed and fabricated for the detection of thrombin, based on the optimization of fixed conditions for the amino aptamer （T29） on aldehyde glass substrate, and fixed on characterization results. Our results showed that the fixation concentration of T29 was 5 uM, the fixation buffer was 3 ＊ SSC containing 37.5% DMSO, and finally realized the detection of thrombin.

关 键 词：适配体 微阵列芯片 凝血酶 

分 类 号：TS207.4[轻工技术与工程—食品科学]