维药小茴香抗肝纤维化作用及对TGF-β/smad信号转导通路的影响  

Effects of foeniculum vulgare mill of anti liver fi brosis on TGF-β/smad signal pathways

作　　者：张泽高[1] 肖琳[1] 詹欣宇[1] 银皓[1] 鲁晓擘[1] 张跃新[1] 

机构地区：[1]新疆医科大学第一附属医院感染科,乌鲁木齐830054

出　　处：《中国肝脏病杂志（电子版）》2014年第1期32-37,共6页Chinese Journal of Liver Diseases：Electronic Version

基　　金：新疆维吾尔自治区自然科学基金(2011211A080)

摘　　要：目的探讨小茴香提取物抗肝纤维化的作用及其对TGF-β/smad信号转导通路和肝星状细胞活化的影响。方法选择44只SD大鼠给予40%四氯化碳(CCl4)橄榄油溶液4 ml/(kg·体重)皮下注射5周制备肝纤维化模型,成模后随机分成治疗组和模型对照组,同时取6只正常大鼠做空白对照。治疗组给予小茴香提取物灌胃,模型对照组给予生理盐水灌胃,4周末处死大鼠检测血清ALT、AST、HA、LN,肝石蜡切片行Masson染色和抗α平滑肌动蛋白(α-SMA)、TGF-β受体Ⅰ型(TGF-βRⅠ)、TGF-β1免疫组织化学染色,反转录实时荧光定量PCR分析肝组织TGF-β1、smad2 mRNA相对内参基因甘油醛-3-磷酸脱氢酶(GAPDH)的表达量。结果小茴香提取物治疗组血清ALT、AST、HA水平,肝组织内胶原纤维含量、α-SMA、TGF-βRⅠ、TGF-β1表达以及TGF-β1、smad2 mRNA相对表达量均明显低于模型对照组。结论小茴香提取物可通过抑制TGF-β/smad信号转导通路抑制肝星状细胞活化,从而减轻大鼠肝纤维化。Objective To evaluate the therapeutic effects of Foeniculum Vulgare Mill in liver ifbrosis rats, and to investigate how it work on TGF-β/smad signal pathways and hepatic stellate cell activation. Methods Total of fifty male SD rats were randomly divided into 3 groups：a blank control group （BC group, 6 rats）, a hepatic ifbrosis model control group （HFC group, 22 rats）, a foeniculum vulgare mill treatment group （FVMT group, 22 rats）. Forty-four rats of HFC group and FVMT group were given 40%CCl4 olive oil mixture by subcutaneous injection to make hepatic ifbrosis models for 5 weeks. Rats in FVMT group were given FVMT by intragastric administration, while rats in HFC group were treated with saline for weeks and then were sacriifced. Serum alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, hyaluronic acid （HA）, laminin （LN） were detected. Histopathologic changes were observed after H.E. and Masson stainings. The expression of alpha-smooth muscle actin （α-SMA） were detected by immunohistochemical staining. TGF-β1, smad2 mRNA were detected by RT-PCR. Results The level of serum ALT, AST, HA, LN and content of collagen fibers, α-SMA, TGF-βRⅠ, TGF-β1 in the liver of FVMT group rats were significantly lower than those of HFC group. The expression of TGF-β1, smad2 mRNA were higher in HFC group than those of FVMT group. Conclusions Uygur Herb foeniculum vulgare mill may reduce liver ifbrosis by suppressing the activation of TGF-β/smad signal pathways and prevent the activation of hepatic stellate cell.

关 键 词：小茴香 肝纤维化 转化生长因子-Β1 信号转导通路 TRANSFORMING growth factor-β1 

分 类 号：R575.2[医药卫生—消化系统]