结直肠癌中FcγRⅡ和VEGF-A的表达与临床意义  

Expression and clinical significance of FcγRⅡ and VEGF-A in colorectal cancer

作　　者：丁清清[1] 管蔚[2] 丁永斌[2] 梁辉[2] 薛绮萍[1] 陈琪[3] 

机构地区：[1]南京医科大学第一附属医院老年医学科,江苏南京210029 [2]南京医科大学第一附属医院普外科,江苏南京210029 [3]南京医科大学病理生理学系,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2014年第2期179-182,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省六大人才高峰资助项目(WSW-027);江苏省高校自然科学研究资助项目(13KJB320012);国家自然科学基金重点项目(81230070)

摘　　要：目的:探讨免疫球蛋白G Fc段低亲和力受体Ⅱ(FcγRⅡ)和表皮生长因子A(VEGF-A)在结直肠癌,发生发展中的作用机制。方法:采用实时荧光定量PCR法,检测FcγRⅡ、VEGF-A基因在56例大肠癌组织和相应切缘正常组织中的表达,分析FcγRⅡ和VEGF-A与患者临床病理特征之间的关系,以及FcγRⅡ与VEGF-A的相关性。结果:FcγRⅡ基因表达在大肠癌组织中较正常组织低,其表达与大肠癌患者的浸润深度和TNM分期相关(均P<0.05)。VEGF-A基因表达在大肠癌组织中较正常组织高,其表达与大肠癌患者的年龄、肿瘤大小、浸润深度和TNM分期相关(均P<0.05)。肠癌患者FcγRⅡ基因的表达与VEGF-A呈负相关(r=-0.104,P=0.002)。结论:FcγRⅡ基因与肠癌的侵袭转移等密切相关,其高表达提示较好的预后,推测可能是通过调控VEGF-A的表达而发挥作用。FcγRⅡ基因有望成为肿瘤免疫治疗的新途径。Objective：To investigate the expression of FcγRⅡFc fragment of IgG,low affinity IIa,receptor（FcγRⅡ） and vascular endothelial growth factor A（VEGF-A） in colorectal cancer and analyze their association,and consequantly explore the mechanism of FcγRⅡin colorectal cancer. Methods：Expression of FcγRⅡand VEGF-A and their association with clinicopathological features were determined using real time polymerase chain reaction（RT-PCR） in 56 cases of colorectal cancer patients. The distribution of the clinicalpathologic and the expression of VEGF-A and FcγR Ⅱ were analyzed using Fisher＇s exact test and the Chi-squared test. Spearman＇s rank correlation was used to analyze the correlation between FcγRⅡand VEGF-A expression. Results：FcγRⅡ mRNA expression in colorectal cancer tissues was significantly lower than that in matched adjacent normal tissues. FcγRII mRNA expression was significantly related to the patients＇ infiltration depth and clinical stage（P 0.05）. VEGF-A mRNA expression in colorectal cancer tissues was significantly higher than that in matched adjacent normal tissues. VEGF-A mRNA expression was significantly related to the patients＇ age,tumor size,infiltration depth and clinical stage（P 0.05）. In colorectal carcinoma, FcγRⅡand VEGF-A mRNA expression was significantly negatively correlated（r =-0.104, P = 0.002）. Conclusion：The expression of VEGF-A and FcγR Ⅱwas associated with the clinical features of colorectal cancer. Expression of FcγRII was negatively correlated with VEGF-A. This study indicates that FcγRⅡand VEGF-A expression is related to the tumor invasion and metastasis. FcγRⅡ may become a new cancer immunotherapy target.

关 键 词：结直肠癌 FcγRII VEGF-A 

分 类 号：R735.3[医药卫生—肿瘤]