SALL4基因在宫颈癌、宫颈上皮内瘤变组织和血浆中表达的研究  

Expression and clinical significance of SALL4 gene in cervical cancer and cervical intraepithelial neoplasia

作　　者：巢浩界 张铭[1] 张一鸣[1] 左伟[2] 周蓓蓓[3] 

机构地区：[1]南京医科大学附属常州妇幼保健院检验科,江苏常州213003 [2]南京医科大学附属常州妇幼保健院病案室,江苏常州213003 [3]南京医科大学附属常州妇幼保健院妇科,江苏常州213003

出　　处：《南京医科大学学报（自然科学版）》2014年第2期188-191,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：常州市科技计划指导性项目(CY20120018);常州市卫生局指导性科技项目(WZ201120)

摘　　要：目的：研究SALL4基因在宫颈癌、宫颈上皮内瘤变（cervical intraepithelial neoplasia,CIN）组织和血浆中的表达情况并探讨其意义。方法：采用实时定量PCR方法检测56例宫颈癌患者、120例CIN患者组织和血浆中SALL4 mRNA的表达,以35例正常宫颈及慢性宫颈炎组织和血浆作为对照,分析宫颈癌组织和血浆中SALL4 mRNA相对表达量的相关性。并用ELISA方法检测各组血浆中SALL4水平。结果：对照组、CINⅠ组、CINⅡ~Ⅲ组、宫颈癌组组织中SALL4 mRNA相对表达水平分别为（4.58±0.64）×10-4、（6.86±0.72）×10-4、（27.03±2.85）×10-4、（40.8±5.50）×10-4,血浆中SALL4 mRNA相对表达水平分别为（0.14±0.05）×10-4、（0.16±0.07）×10-4、（0.49±0.08）×10-4、（2.18±0.22）×10-4,血浆中SALL4的浓度分别为（237±45）μg/L、（256±59）μg/L、（713±62）μg/L、（1 135±107）μg/L,随着病变的逐步进展,SALL4的表达逐渐增强,其中SALL4在宫颈癌组中的表达显著增强,与其他各组比较,差异有统计学意义（P〈0.01）.宫颈癌组组织和血浆中SALL4 mRNA的相对表达水平正相关（r=0.684,P〈0.01）。结论：SALL4基因在CIN、宫颈癌组织和血浆中表达上调,可能在宫颈癌发生、发展中起一定作用。Objective：To investigate the expression pattern and significance of SALL4 gene in CIN and cervical cancer. Methods： The expression of SALL4 mRNA in tissues and plasma of 56 cases of cervical cancer,120 cases of CIN,35 cases of normal cervical or chronic cervicitis（control group） was detected by Real-time PCR. The correlation analysis was conducted to assess the relationship between SALL4 expression in tissues and plasma of cervical cancer group. The levels of SALL4 in plasma were detected by enzyme linked immunosorbent assay. Results：The tissue levels of SALL4 mRNA expression in the the control group,CINⅠ group,CINⅡ~Ⅲ group and cervical cancer group were（4.58 ± 0.64） ×10-4,（6.86 ± 0.72）×10-4,（27.03 ± 2.85）×10-4and（40.8 ± 5.50）×10-4. The plasma levels of SALL4 mRNA expression were（0.14 ± 0.05）×10-4,（0.16 ± 0.07）×10-4,（0.49 ± 0.08）×10-4and（2.18 ± 0.22）×10-4. The concentration of SALL4 in plasma were（237 ± 45）μg / L,（256 ± 59）μg / L,（713 ± 62）μg / L and（1 135 ± 107）μg / L, respectively. With the progress of cervical disease,the expression of SALL4 was increasing gradually,the expression of SALL4 in cervical cancer group was higher than that in other groups（P 0.01）. A positive correlation between SALL4 expression in tissues and plasma was observed in cervical cancer group（r = 0.684,P 0.01）.Conclusion： SALL4 expression was up-regulated in the CIN and cervical cancer tissues and plasma,and it may be probably associated with the initiation,progression of cervical cancer..

关 键 词：SALL4基因 宫颈癌 宫颈上皮内瘤变 实时定量PCR 酶联免疫吸附试验 

分 类 号：R737[医药卫生—肿瘤]