游离脂肪酸抑制TRAIL诱导的活化肝星状细胞凋亡  

Free fatty acids decrease the apoptosis of activated hepatic stellate cells induced by TRAIL

作　　者：胡彬[1,2] 王赫[1,2] 徐会[1,2] 王钧毅 赵娜[1,2] 杨俊涛 

机构地区：[1]蛋白质组学国家重点实验室,北京蛋白质组研究中心,国家蛋白质科学中心 [2]军事医学科学院放射与辐射医学研究所,蛋白质药物国家工程研究中心,北京102206

出　　处：《肝脏》2014年第2期104-107,共4页Chinese Hepatology

基　　金：国家自然科学基金资助项目(31000405);国家高技术研究发展计划(2012AA020201)

摘　　要：目的鉴定游离脂肪酸(free fatty acids,FFA)对肝星状细胞(HSC)凋亡的影响。方法体外培养活化人HSC LX-2,加入FFA处理后,TRAIL诱导凋亡,通过流式细胞术检测细胞凋亡情况。通过caspase-3活性检测试剂盒检测细胞内caspase-3的活化情况,通过Q-PCR检测细胞内凋亡相关基因的表达情况。结果 FFA能够抑制TRAIL诱导的活化LX-2细胞凋亡,降低细胞内caspase-3的活性,Q-PCR的分析表明,FFA能够影响细胞内凋亡相关基因BAX的表达,下调死亡受体TRAIL-R2和FAS的表达。结论体外FFA处理,能够抑制活化HSC的凋亡。Objective To explore the effect of free fatty acids （FFAs） on the apoptosis of cultured hepatic stellate cells .Methods Activated human hepatic stellate cell （LX-2） line was induced apoptosis by TRAIL after being treated with FFAs .Apoptosis was detected by FACS .Intracellular caspase-3 activity was determined by caspase-3 assay kit . The expression of cell apoptosis-related genes was analyzed by real time-PCR .Results FFAs inhibited the apoptosis of activated LX-2 cell induced by TRAIL .Cellular activity of caspase3 was decreased by FFAs treatment .Q-PCR analysis showed that FFAs could decrease the expression of BAX , FAS and TRAIL-R2 . Conclusion FFAs could inhibit apoptosis of activated hepatic stellate cells in v itro .

关 键 词：游离脂肪酸 肝星形细胞 肝纤维化 

分 类 号：R575[医药卫生—消化系统]