食管鳞癌组织MTA2表达与淋巴管生成关系探讨  

Correlation between expression of MTA2 and lymphangiogenesis in esophageal squamous cell carcinoma

作　　者：李秀娟[1] 李明霞[2] 赵轶峰[3] 金春亭[1] 范婕[1] 李海军[1] 李玉珍[1] 

机构地区：[1]河北北方学院病理教研室,河北张家口075000 [2]河北北方学院附属第一医院内分泌科,河北张家口075000 [3]河北北方学院附属第一医院肿瘤外科,河北张家口075000

出　　处：《中华肿瘤防治杂志》2014年第9期674-677,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：河北省高等学校科学技术研究青年基金项目(QN20131156)

摘　　要：目的：探讨食管鳞癌组织中MTA2表达与淋巴管生成的关系。方法：收集1995—01—01—2010—12—31河北北方学院附属第一医院手术切除的食管癌标本96例及正常黏膜标本（距离肿瘤组织〉5cm）35例，应用免疫组织化学SP法检测食管鳞癌及癌旁正常黏膜组织中MTA2蛋白的表达，淋巴管内皮特异性标志Podoplanin检测食管鳞癌及正常黏膜组织中的淋巴管密度（lymphatic vessel density，LVD），分析MTA2表达及LVD与各临床病理指标的关系，探讨两者之间的相关性。Cox回归模型评估MTA2、LVD及各病理指标与患者预后的关系。结果：96例食管鳞癌组织中MTA2阳性表达率为65．6％（63／96），且MTA2在高、中、低分化组的阳性表达率分别为48．0％（12／25）、60．9％（14／23）和77．1％（37／48），在淋巴结转移组和无淋巴结转移组的阳性率分别为77．2％（44／57）和48．7％（19／39），在I～Ⅱ期和llI～IV期阳性率分别为55．3％（26／47）和75．5％（37／49），MTA2表达与分化程度（X2=6．467，P=0．039）、淋巴结转移（X2=8．323，P=0．004）和TNM分期（X2=4．335，P=0．037）明显相关。死亡病例MTA2的阳性表达率74．6％（50／67）也明显高于存活病例的44．8％（13／29），X2=7．967，P=0．005。96例食管鳞癌组织LVD为6．07±1．95，显著高于正常组织的4．78±1．35，P=0．001；淋巴结转移组的LVD为8．25±1．86，也明显高于无淋巴结转移组的4．72±1．18，P=0．004；死亡病例的LVD为7．61±2．22，存活病例的为4．95±1．55，差异有统计学意义，P=0．028。食管鳞癌组织中MTA2阳性和阴性表达的LVD分别为（7．87±2．09）和（4．79±1．35），差异有统计学意义，P=0．009。Cox模型多因素分析结果表明，分化程度、淋巴结转移和LVD均是影响食管癌患者生存的独立因素，P〈0．05。结论：MTA2的阳性表达可能是诱导食管癌淋巴管OBJECTIVE：To study the expression and interaction of MTA2 and Podoplanin in esophageal squamous cell carcinoma （ESCC）, as well as the correlation with lymphangiogenesis. METHODS： From 1995-01-01 to 2010-12-31, 96 cases of ESCC and 35 normal esophageal mucosa （distance from tumor tissue 〉5 cm） were collected in the pathology department of the First Hospital Affiliated to Hebei North College. Immunohistochemistry staining SP was used to detect the expression level of MTA2. Using podoplanin as the specific marker of lymphatic endothelium,lymphatic vessel density （LVD） in ESCC and normal esophageal mucosa were calculated. Subsequently we analyzed the relationship between postoperative pathological features with the expression of MTA2 and Podoplanin. Cox regression analysis was performed to e- valuate the connection between expression of MTA2 and LVD and prognosis. RESULTS： The MTA2 was positively expressed in 63 cases of ESCC（65.6%, 63/96）. The rates of MTA2 among well, moderately and poor differentiated ESCC were 48.0%（12/25） ,60.9%（14/23） and 77.1%（37/48）. The positive rates of MTA2 were 77.2%（44/57） and 48.7% （19/39） in positive and negative lymph mode metastasis. The positive rates in I -II and III -IV groups were 55.3% （26/47） and 75.5% （37/49）. The MTA2 expression in ESCC had significant relations with differentiation（X2= 6. 467, P=0. 039） ,lymph mode metastasis （X2 =8. 323,P=0. 004） and TNM stage （X2=4. 335,P=0. 037）. The expression of MTA2 of deaths（74.6%,50/67） was higher than that of the survival cases（44.8%,13/29）（X2 =7. 967,P=0. 005）. The LVD in ESCC （6.07±1.95） was obviously higher than that in normal esophageal mucosa（4. 78±1. 35） （P = 0.001 ）, and had significant relation with lymph mode metastasis （8.25±1.86 vs 4.72±1.18,P=0. 004）. The LVD was significantly different between the deaths （7.61±2.22） and survival （4. 95 ± 1. 55） cases （P =0. 028）. The LVD was significantly higher in 63 ESCC （7.

关 键 词：食管肿瘤 MTA2 PODOPLANIN 淋巴管生成 淋巴管密度 免疫组织化学 

分 类 号：R735.1[医药卫生—肿瘤]