好氧反硝化细菌N22’种子培养基的优化  

Optimization of Seed Culture Medium of Aerobic Denitrification Bacteria N22'

作　　者：郭端强[1] 王孝娟[1] 尚亚君 王美姿 单林娜[1] 

机构地区：[1]河南城建学院生命科学与工程学院,河南平顶山467036

出　　处：《生物技术》2014年第2期80-84,共5页Biotechnology

基　　金：河南省科技攻关重点项目("净化城市景观富营养化水体的微生物菌剂研究";No.092102310069)资助

摘　　要：目的:优化好氧反硝化细菌N22’的种子培养基,提高对数期末期细菌浓度。方法:采用Plackett-Burman设计对影响N22’细菌浓度的因素进行评估并筛选出具有显著效应的因素KNO3(X3)、KH2PO4(X4)和K2HPO4(X5),经过最陡爬坡实验接近3个因素的最大响应区域后,应用Box-Behnken设计和响应面分析法确定3个因素的最优水平。结果:优化后的种子培养基:柠檬酸钠6g,KNO32.72g,KH2PO41.35g,K2HPO41.12g,MgSO4·7H2O 0.25g,CaCl20.025g/L,FeSO4·7H2O 0.025g,EDTA0.125g/L;蒸馏水1 000mL,初始pH值7.0。优化后发酵液对数期末期细菌浓度达到1.684 0×1012cfu/mL,比优化前1.632 7×1011cfu/mL提高了9.31倍。结论:Plackett-Burman设计结合响应面分析方法优化了菌株N22’的种子培养基。Objective： The seed culture medium was optimized to improve the bacterial concentration of strain N22＇. Method： Firstly, the effects of different factors on bacterial concentration in the seed culture medium was investigated by Plackett - Burman design, Statistically significant factors are ： KNO3 （ X3 ）, KH2 PO4 （ X4 ） and K2 HPO4 （ X5 ）. Then, the steepest ascent experiment was adopted to approach opti- mal region for the three factors. Finally, Box - Behnken design and response surface methodology was used to identify the optimal level of the three factors. Result ：The optimal seed culture medium is： sodium citrate 6g, KNO3 2. 72g, KH2PO4 1.35g, K2HPO4 1.12g, MgSO4 · 7H2 0 0.25g, CaCl2 0. 025g, FeSO4 · 7H2O 0. 025g, EDTA 0. 125g;distilled water 1 000mL,initial pH value 7. 0. The bacterial con-centration reached 1.684 0 x 1012 cfu/mL at the telophase of logarithmic phase after optimization, which increased 931% than before. Condusion： The seed culture medium of strain N22＇ can be optimized through Plackett - Burman design combined with response surface analysis.

关 键 词：好氧反硝化细菌 优化 种子培养基 Plackett—Burman设计 最陡爬坡实验法 Box—Behnken设计 响应面法 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]