核酸适配体修饰的铁纳米粒对HER2阳性乳腺癌的靶向热疗效应  

Enhancement of thermal damage to HER2-positive breast cancer cells by aptamer-guided magnetic nanoparticles

作　　者：李凤武[1] 胡燕[1] 段金虹[1] 许海燕[2] 杨先达[1] 

机构地区：[1]中国医学科学院北京协和医学院基础医学研究所病理与病理生理系,北京100005 [2]中国医学科学院北京协和医学院基础医学研究所生物医学工程系,北京100005

出　　处：《基础医学与临床》2014年第5期644-647,共4页Basic and Clinical Medicine

基　　金：科技部重大科学计划(2011CB933504;2011CB911004;2011CB911003)

摘　　要：目的研发能提高HER2阳性乳腺癌热疗效应的核酸适配体-铁纳米粒子复合体(AptNPs),探索肿瘤靶向治疗的全新途径。方法利用生物素和链霉亲和素的结合反应构建AptNPs,用动态光散射粒径仪表征其尺寸分布,用流式细胞仪和相差显微镜检测AptNPs的靶向结合能力,MTS法测其对磁场下抗肿瘤热疗效果的影响。结果通过生物素-亲和素反应构建了AptNPs,其平均直径为333.7 nm,对HER2阳性细胞有较强的结合,对HER2阴性细胞没有结合。此外,AptNPs可显著提升对HER2阳性细胞的热疗杀伤率(P<0.05),但对HER2阴性细胞的热疗后杀伤率影响较小。结论 AptNPs能靶向性地提升针对HER2阳性肿瘤细胞的热疗效率,在研发新型肿瘤靶向热疗方面具有潜在应用价值。Objective To develop aptamer-modified nanoparticles （AptNPs） for targeted enhancement of thermal damage to HER2-positive breast cancer cells.Methods HER2 aptamer was connected to NPs via biotin-streptavidin reaction.AptNPs were characterized by Dynamic Light Scattering （DLS）.The binding feature of the aptamer was evaluated by flow cytometry,and the affinity of AptNPs to target cells by phase-contrast microscopy.Thermal damage under alternative magnetic field was measured by MTS assay.Results The average size of AptNPs was 333.7 nm.AptNPs exhibited strong binding to the HER2-positive but not the HER2-negative cells.Importantly,AptNPs enhanced the thermal damage to the HER2-positive tumor cells,but not that to the HER2-negative cells.Conclusions Aptamer-guided iron particles may have potential utility in development of novel HER2-targeted thermal therapies.

关 键 词：核酸适配体 热疗 HER2阳性 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]