血链球菌细菌素对念珠菌细胞表面疏水性和细胞膜钙离子通透性的影响  

Study of Cell Surface Hydrophobicity and Calciumion Permeability of Cell Membrane of Sanguis Bacteriocin on Candida

作　　者：马晟利[1] 夏雪[2] 董雪[2] 葛文玉 赵英男[2] 

机构地区：[1]黑龙江省医院口腔科,黑龙江哈尔滨150036 [2]佳木斯大学佳木斯大学口腔医学院

出　　处：《口腔医学研究》2014年第4期321-325,共5页Journal of Oral Science Research

基　　金：黑龙江省自然科学基金资助项目(D2007-03)

摘　　要：目的:研究血链球菌细菌素对单独及混合培养的白色念珠菌(C.a)及热带念珠菌(C.t)细胞表面疏水性的影响。研究血链球菌细菌素对单独及混合培养的白色念珠菌(C.a)及热带念珠菌(C.t)细胞膜钙离子通透性的影响。方法:复苏并传代血链球菌标准株ATCC10556,通过低温高速离心,细胞破碎等方法提取血链素,使其作用于1×106 cfu/mL单独及混合培养的C.a及C.t,于37℃,80r/min振荡培养。1)采用二倍稀释法,测定血链素对C.a及C.t最小抑制浓度(MIC);2)运用微生物黏着碳氢化合物的方法[1],用紫外分光光度计测A值,计算C.a及C.t的疏水率;3)分别向单独及混合培养的C.a及C.t菌悬液中装载钙离子荧光探针(Fluo-3AM),利用激光共聚焦显微镜观察血链素作用后的C.a及C.t细胞内钙离子浓度的变化。结果:血链素对单独培养的C.a及C.t的MIC为0.25g/L;血链素对等量混合培养的C.a及C.t的MIC为0.5g/L。2)血链素作用后,单独及混合培养的C.a及C.t细胞表面疏水率均明显降低,与对照组相比具有显著差异(P<0.05)。在血链素的作用下,单独及混合培养的C.a及C.t的细胞内钙离子浓度均明显降低,与对照组相比具有统计学意义(P<0.05)。结论:血链素可以导致白色念珠菌及热带念珠菌细胞表面疏水性降低。血链素可以导致白色念珠菌及热带念珠菌细胞膜钙离子通透性增强。Objective： To study the cell surface hydrophobicity and membrane permeability of Streptococcus san- guis bacteriocin on Candida albicans（C, a） and Candida tropicalis （C. t）. Methods： Recovery and extend standard strains ATCC10556,Streptococcus sanguis bacteriocin was extracted by low--temperature high--speed centrifuga- tion, ultrasonic disruption, and then was respectively applied to separate and mixed cultured C. a and C. t under 37℃ 80r/min oscillating culture. 1. Used Adopt double dilution method to determine the MIC of C. a and C. t in un- der the action of Streptococcus sanguis bacteriocin. 2. Using the method of microbial gelling hydrocarbons, measuring absorbance value with Ultraviolet spectrophotometer and calculating hrdrophobic. 3. Adding the Fluo--3 AM to C. a and C. t and observing concentration of calcium ion into cell with Laser scanning confocal microscope. Results： The MIC of separate C. a and C. t is 0.25g/L, The MIC of mixed C. a and C. t is 0.5g/L. Under the influence of Sanguis Bacteriocin hrdrophobic of C. a and C. t reducing significantly, compared with the control group have significant difference. 3. Under the influence of Sanguis Bacteriocin concentration of calcium ion into cell of C. a and C. t de- creased significantly,compared with the control group have significant difference. Conclusion： Streptococcus sanguis bacteriocin could significantly inhibit cell surface hrdrophobicity of separate and mixed cultured C. a and C. t. Strep- tococcus sanguis baeteriocin enhanced the calcium ion permeability of cell membrane of C. a and C. t.

关 键 词：血链球菌细菌素 白色念珠菌 热带念珠菌 细胞表面疏水性 细胞膜钙离子通透性 

分 类 号：R780.2[医药卫生—口腔医学]