DADS抑制食管胃交界部腺癌细胞OE19增殖及诱导凋亡的机制  

The effect and mechanism of DADS inhibiting the proliferation and inducing apoptosis of gastro-esophageal adenocarcinoma cell line OE19

作　　者：尹晓然[1] 冯诚[2] 张军[2] 马红兵[1] 王西京[1] 张淑群[1] 焦杨[3] 张蓉[2] 

机构地区：[1]西安交通大学医学院第二附属医院肿瘤科,陕西西安710004 [2]西安交通大学医学院第二附属医院消化科,陕西西安710004 [3]西安交通大学医学院第二附属医院内分泌科,陕西西安710004

出　　处：《西安交通大学学报（医学版）》2014年第3期370-374,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省社发攻关项目(No.2010k01-141)~~

摘　　要：目的探讨二烯丙基二硫化物(DADS)体外抑制食管胃交界部腺癌细胞株OE19增殖并诱导凋亡的作用及相关机制。方法 DADS处理OE19,倒置显微镜下观察细胞形态变化;MTT检测DADS对OE19细胞的增殖抑制作用;使用流式细胞术分析不同浓度DADS处理OE19后的凋亡率;Real-time PCR检测DADS对OE-19细胞Caspase-3、Caspase-9、Bcl-2、Bax以及NF-κB mRNA表达水平的影响。结果作用24、48h后,DADS可以抑制OE19增殖,抑制作用呈剂量依赖性。使用40、80μg/mL的DADS处理OE19细胞24、48h,凋亡细胞比例分别为14.0%、25.4%和19.0%、27.2%。Real-time PCR检测发现DADS能够上调OE19细胞Caspase-3、Caspase-9mRNA的表达,并且能够下调NF-κB、Bcl-2mRNA的表达,且呈剂量-反应关系,Bcl-2/Bax的比值明显降低。结论 DADS在体外能够抑制食管胃交界部腺癌细胞的增殖并诱导其凋亡,其凋亡机制可能是通过线粒体途径,同时NF-κB通路以及Bcl-2家族成员均参与了该过程。Objective To investigate the effect and mechanism of DADS in inhibiting the proliferation and inducing apoptosis of gastro-esophageal cancer cells in vitro. Methods The gastro-esophageal adenocarcinoma cells OE19 were treated by DADS of different concentrations in vitro. Morphologic changes were observed by the microscope and MTT assay was performed to test the growth-inhibitory effect of DADS on OE19 cells. Apoptosis rate of OE19 treated with different concentrations of DADS was measured by flow cytometry. Real-time PCR was used to detect DADS-induced effects on mRNA expressions of Caspase-3, Caspase-9, Bcl-2, Bax and NF-kB in OE19 cells. Results DADS inhibited the proliferation of OE19 cells in a dose-dependent manner. The apoptosis rate of OE19 cells was 14.0%, 25.4% and 19.0% and 27.2%, respectively, when treated with 40 and 80μg/mL DADS for 24 h and 48 h. Real-time PCR assay showed that DADS could enhance mRNA expression levels of Caspase-3 and Caspase-9 and significantly decrease the mRNA expression levels of NF-kB and Bcl-2 and the ratio of Bcl-2/Bax. Conclusion DADS can significantly inhibit the proliferation and induce the apoptosis of gastro-esophageal adenocarcinoma cells via mitochondria-dependent pathways, which may be related to NF-kB and Bcl-2 families.

关 键 词：二烯丙基二硫化物(DADS) 食管胃交界部腺癌 增殖 凋亡 OE19 

分 类 号：R735[医药卫生—肿瘤]