胃癌组织中神经纤毛蛋白2表达与肿瘤淋巴管新生和临床病理因素的相关性  

Correlation between the expression of neuropilin-2 and tumor-associated lymphangiogenesis in gastric cancer and clinicopathological parameters

作　　者：徐卫国[1] 刘云[1] 贾纯亮[2] 苗娜[1] 徐洪[3] 王宇鹏[5] 张世伟[1] 孙红[4] 刘和亮[3] 

机构地区：[1]河北联合大学附属医院肿瘤外科,河北省唐山市063000 [2]唐山市人民医院胃肠外科 [3]河北联合大学医学实验研究中心 [4]河北联合大学基础医学院病理学系 [5]天津市北辰医院肿瘤科

出　　处：《中华医学杂志》2014年第14期1067-1070,共4页National Medical Journal of China

基　　金：河北省科学技术研究与发展计划（10276115D）

摘　　要：目的：探讨神经纤毛蛋白2（NRP2）在胃癌组织中的表达及其与微淋巴管新生及临床病理因素的相关性。方法回顾性分析河北联合大学附属医院和唐山市人民医院2011年10月至2013年10月手术切除胃癌组织标本（每例患者分别取癌组织、癌旁组织及相对正常区组织）共65例资料，采用免疫组织化学法检测NRP2表达情况，选取D2-40单克隆抗体标记淋巴管，计数微淋巴管密度（ MLD），观测淋巴管形态。结果癌组织、癌旁组织及相对正常区组织各65例，其中胃癌组织NRP2阳性表达率高于癌旁组织和正常组织[61.5％（40／65）比49.2％（32／65）和4.6％（3／65），均P＜0.05]；胃癌组织和正常组织MLD值分别为（12±5）、（23±6）个／mm2，均低于癌旁组织[（44±13）个／mm2，均P＜0.05]，各组淋巴管形态也各不相同；同时癌旁NRP2的表达情况及MLD与肿瘤大小、细胞分化程度、组织浸润深度、淋巴管浸润、淋巴结转移及肿瘤TNM分期均相关（均P＜0.05）。NRP2的表达强度与MLD呈正相关（r＝0.517，P＝0.000）。结论 NRP2可能参与调节胃癌微淋巴管新生和淋巴道转移，在胃癌肿瘤发生、病期进展中具有关键作用，推断其可作为胃癌预后判断的相关指标。Objective To explore the correlation between the expression of neuropilin-2 （ NRP2 ） and tumor-associated lymphangiogenesis and clinicopathological parameters in gastric carcinoma （ GC ）.Methods All GC, margin and normal tissues of 65 cases through surgical resection were retrospectively collected at our hospital and Tangshan People′s Hospital from October 2011 to October 2013.The expressions of NRP2 in GC, tumor margin and normal gastric tissues were analyzed by immunohistochemistry.Lymphangiogenesis was marked with D 2-40 monoclonal antibody and microlymphatic density （ MLD） counted.And the morphology of lymphatic vessels was observed.Results A positive expression of NRP2 was found in GC （ n=40, 61.5%） and it was significantly higher than that in tumor margin （n=32, 49.2%） and normal tissues （n=3, 4.6%） （P〈0.05）.But the counts of MLD in both GC （12 ±5）/mm2 and normal region （23 ±6）/mm2 were significantly lower than that of tumor margin region （44 ±13）/mm2（P〈0.05）.In addition, the morphologies of lymphatic vessels were different among 3 groups.Also NRP2 expression and MLD count were correlated with differentiation degree , infiltrative degree, lymphatic vessel invasion, lymph node metastasis and TNM staging of GC （all P〈0.05）.The expression intensity of NRP2 in tumor margin tissues was positively correlated with MLD （ r=0.517, P=0.000）.Conclusions The expression of NRP2 in GC may modulate lymphangiogenesis and lymphatic metastasis.And it may play a key role in tumorigenesis , progression and prognosis predicating in GC.

关 键 词：胃肿瘤 神经纤毛蛋白质2 淋巴转移 淋巴管新生 Neuropilin-2 

分 类 号：R735.2[医药卫生—肿瘤]