硇洲岛褐蓝子鱼肠道抗菌活性菌株的分离及多样性  

Diversity of antimicrobial bacteria associated with Siganus fuscescens from Naozhou island in Zhanjiang,China

作　　者：刘颖[1] 时威 安俊莹[1] 赵鸭美 叶日英[1] 徐春厚[4] 

机构地区：[1]广东海洋大学食品科技学院,广东湛江524088 [2]广东环凯微生物科技技有限公司,广东广州510663 [3]浙江湖州环境科技创新中心,浙江湖州313028 [4]广东海洋大学农学院,广东湛江524088

出　　处：《微生物学报》2014年第5期582-588,共7页Acta Microbiologica Sinica

基　　金：广东省科学技术厅项目(2010B020313002;S2011010000328;粤科规划字[2009]198号)~~

摘　　要：【目的】了解广东湛江硇洲岛褐蓝子鱼(Siganus fuscescen)肠道抗菌物质产生菌的多样性。【方法】采用传统的分离方法与牛津杯扩散法对褐蓝子鱼肠道菌进行分离纯化与抗菌活性测定,运用16S rRNA基因序列的系统发育分析对抗菌活性菌株进行多样性分析。【结果】根据形态观察和部分生理生化实验去冗余,从褐蓝子鱼肠道样品中分离到68株细菌,其中抗菌活性菌株19株,占分离株的27.9%。19株不同种类的活性菌株分属于细菌域的放线杆菌门(Actinobacteria)、变形杆菌门(Proteobacteria)与厚壁菌门(Firmicutes)的11个科、12个属。多数菌株属于厚壁菌门(8株,42.1%),其次是变形杆菌门(7株,36.8%)和放线杆菌门(4株,21.1%)。大多数抗菌活性菌株与其系统发育关系最密切的已知典型菌株之间存在一定的遗传差异(16S rRNA基因序列相似性为96.2%-99.9%),其中有4株代表潜在的新种。【结论】广东湛江硇洲岛褐蓝子鱼肠道中存在较为丰富的细菌抗菌物质产生菌,并蕴藏着较多的微生物新类群。[ Objective] Marine bacteria are a rich source of potentially useful antimicrobial molecules. The purpose of the study is to explore the diversity of bacteria with antimicrobial activity isolated from Siganus fuscescens gastrointestinal tract collected from Naozhou Island （20° 52＇ N- 20° 56＇ N 110° 33＇ E -110° 38＇ E）, Leizhou Bay, South China Sea. [ Methods] We isolated bacteria from the gastrointestinal tract of fish sample using classical culturing technique, and determined antimicrobial activities of the isolates by Oxford cup method. We investigated diversity of antimicrobial isolates using phylogenetic comparative analysis of 16S rRNA gene sequences. [ Results] According to the results of morphological observation and part of physiological and biochemical experiments, we isolated 68 strains from fish gastrointestinal tract. Among them, 19 strains with antimicrobial activities were acquired （27.9 % of the isolates） and represented 19 different species, belonging to 12 genera （Rothia, Micrococcu, Brachybacterium, Brevibacterium, Psychrobacter, Paracoccus, Cobetia , Citrobacter , Pseudomonas , Bacillus, Lactobacillus and Staphylococcus ） of 11 families （ Microbacteriaceae, Dermabacteraceae, Brevibacteriaceae, Moraxellaceae, Rhodobacteraceae, Halomonadaceae, Enterobacteriaceae, Pseudomonadaceae, Bacillaceae, Lactobacillaceae and Staphylococcaceae） in three phyla （Actinobacteria, Proteobacteria and Firmicutes）. Eight strains （42. 1% ） , 7 strains （36.8 % ） and 4 strains （21.1% ） were belonging to the phylum Firmicutes, Proteobacteria and Actinobacteria, respectively. The phylogenetic distance matrix resuhs suggested that there were obvious genetic divergences between the majority of strains with antimicrobial activity and their phylogenetically most closely related typical strain, due to 16S rRNA gene sequences similarities ranging from 96.2 to 99.9 %. In addition, 4 strains （ZJHD2-31, ZJHDS-23, ZJHD2-58 and M26） could represent potential new species, and identifica

关 键 词：褐蓝子鱼 抗菌活性 16S RRNA基因 多样性 

分 类 号：S941.4[农业科学—水产养殖]