脂联素通过增强糖尿病大鼠心肌缺血/再灌注损伤后心肌抗氧化能力减少细胞凋亡  

Adiponectin reduces apoptosis by improving myocardial anti-oxidative activity after myocardial ischemia /reperfusion injury in diabetic rats

作　　者：郭佳[1] 边云飞[2] 肖传实[1] 李志东[3] 

机构地区：[1]山西医科大学第一医院心内科,山西太原030001 [2]山西医科大学第二医院心内科,山西太原030001 [3]山西医科大学药理学教研室,山西太原030001

出　　处：《中国药理学通报》2014年第5期623-627,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81170198);山西省研究生优秀创新项目

摘　　要：目的研究脂联素(adiponectin,APN)对糖尿病大鼠心肌缺血/再灌注(ischemia/reperfusion,IR)损伤的保护作用,并从氧化-抗氧化角度初步探讨其作用机制。方法 Sprague Dawley大鼠随机分为糖尿病组和非糖尿病组。糖尿病组腹腔注射链脲佐菌素致糖尿病。非糖尿病组和糖尿病组大鼠被随机分为正常组(NS)、缺血/再灌注组(NIR)、糖尿病组(DS)、DS+IR组(DIR)、DS+IR+APN组(APN)。NIR组、DIR组、APN组动物经历IR损伤,APN组于再灌注前10 min经舌静脉注射APN。结果糖尿病模型组动物血糖明显升高,体重下降(P<0.05)。NIR组和DIR组心梗面积增加,细胞凋亡增多;心肌组织MDA含量、ROS生成均明显高于各自的对照NS组和DS组;SOD、NO活性则分别明显减低(P<0.05或P<0.01);并且DIR组上述指标变化较NIR组更明显。APN预处理可明显逆转DIR所致的心肌凋亡,并抑制氧化应激(P<0.05或P<0.01)。结论 APN可能通过增强抗氧化能力减轻糖尿病大鼠心肌IR损伤。Aim To study the effect of adiponectin （APN） on myocardial ischemia reperfusion （IR） injury in diabetic rats and to explore the role of oxidation-an- tioxidation system. Methods Male Sprague Dawley rats were randomly divided into control group （ NS）, IR group （ NIR ）, diabetes group （ DS ）, DS ＋ IR group （ DIR）, DS ＋ APN ＋ IR group （APN）. Experimental diabetes was induced in the animals by a single intraperitoneal injection of streptozotocin at a dose of 55 mg . kg-1. The IR and NIR group were subjected to myocardial I/R injury. APN group was administered APN through intravenous injection 10 min before the reperfusion, the others were administered normal saline. The rats were considered diabetic and used for the study only if their glucose levels were higher than 15 mmol . L-1. Results All the diabetic rats exhibited increased levels of blood glucose and reduction of body weight （P 〈 0. 05 ）. Compared with those of NS and DS groups, the myocardial infarct size, cardiomyocyte apoptosis, MDA concentration and ROS in NIR and DIR groups were remarkably increased, activities of SOD and NO were decreased（P 〈0. 05 or P 〈0. 01 ）. APN decreased oxidative stress product generation and myocardial apoptosis induced by diabetic myocardial I/ R injury （P 〈 0. 05 ）. Conclusion APN exerts protective effect on myocardial I/R injury in diabetic rats through anti-oxidative mechanism.

关 键 词：脂联素 大鼠 糖尿病 缺血 再灌注损伤 心肌细胞 抗氧化能力 

分 类 号：R332[医药卫生—人体生理学] R322.11[医药卫生—基础医学]