青刺果总黄酮对四氧嘧啶致糖尿病小鼠肾组织形态学的影响  

Effects of flavonoids from Prinsepia utilis Royle on the histomorphology of kidney in alloxan-induced diabetic mice

作　　者：吕程[1] 吴小兰[1] 殷中琼[1] 景波[1] 李正文[1] 戴书俊[1] 

机构地区：[1]四川农业大学动物医学院药学系,四川雅安625014

出　　处：《中国药理学通报》2014年第5期672-675,共4页Chinese Pharmacological Bulletin

基　　金：教育部<长江学者和创新团队发展计划>创新团队项目(No IRTO848)

摘　　要：目的观察青刺果总黄酮对四氧嘧啶导致的糖尿病小鼠肾组织形态学的影响,探讨其对肾脏的保护作用。方法腹腔注射四氧嘧啶建立糖尿病小鼠模型,连续4周给予青刺果总黄酮灌胃治疗。2周及4周后分别处死小鼠,测血糖,称肾重和计算肾脏脏器指数。取部分肾做切片,分别在光镜和电镜下观察肾脏病理变化。结果糖尿病模型小鼠经青刺果总黄酮治疗后,在光镜和电镜下均可观察到由四氧嘧啶导致的肾脏病变的减轻。经青刺果总黄酮治疗的糖尿病小鼠其肾小球体积减小,基底膜无明显增厚;肾小管内糖原沉积减少,间质纤维减少;细胞器损伤较模型组轻微。结论青刺果总黄酮能有效的改善糖尿病小鼠肾脏的病变,控制肾脏肥大,对糖尿病肾病有一定的保护作用。Aim To investigate the effect of flavonoids from Prinsepia utilis Royle（FPR） on the histomorphology of kidney in diabetic mice, and to investigate its protective mechanism. Methods Diabetic mice induced by alloxan were given FPR orally each day for four weeks. After the administration for two and four weeks, ten mice in each group were randomly sacri- riced. The kidneys were removed and weighed. The extracted renal tissue was embedded with paraffin and sectioned, the sections were stained with Hematoxylin and Eosin （ HE ）, Periodic acid Schiff （ PAS ） and Gomoil, and then observed under the microscopy, 1mm3 of renal cortex fixed with glutaral in four centi-degree , and then the ultrastructure of each group was observed under the electron microscope respectively after four weeks＇ treatment. Results Compared with the model control group, in the treatment group, observation under the microscopy showed that glomerular volume and mesangial cells reduced, FPR could relieve thickening of the glomerular basement membrane （ GBM ）, little inflammatory cells infiltrated in the interstitium, tubular epithelial cells almost became normal, renal tubule had little glucogen, fiber decreased in the interstitium of renal tubule. Observation under the electron microscope indicated that foot process in podocytes lined up in order, mitochondria of the renal tubule＇ s epithelial cell almost recovered. Conclusion FPR can relieve the changes of renal pathology, improve renal function, and delay the progression of pathologic changes of kidney in diabetic mice partly through reducing the blood glucose and the blood lipid.

关 键 词：青刺果总黄酮 糖尿病 血糖 肾脏 肾小球 组织形 态 

分 类 号：R-332[医药卫生] R284.1