中华眼镜蛇神经毒素微球的制备及镇痛作用研究  

Preparation and analgesic effect of α-cobrotoxin-loaded poly( lactide-co-glycolide) microspheres

作　　者：郝永龙[1] 陈美荣[2] 黄铭[1] 

机构地区：[1]山东中医药大学第二附属医院药剂科,山东济南250001 [2]山东中医药大学附属医院眼科,山东济南250001

出　　处：《中国药理学通报》2014年第5期676-679,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 30472230)

摘　　要：目的研究中华眼镜蛇神经毒素PLGA微球制备及通过鼻黏膜给药的镇痛作用。方法采用复乳溶剂挥发法制备眼镜蛇神经毒素PLGA微球,采用大鼠甩尾法测定其镇痛效果。结果采用W/O/O方法,成功制备出中华眼镜蛇神经毒素鼻腔给药PLGA微球,微球平均粒径25μm左右,药物包埋率达80%以上。通过大鼠甩尾法显示明显的镇痛效果。结论将中华眼镜蛇神经毒素制成微球制剂鼻腔给药,镇痛作用明显增强。Aim To study the preparation of ot-cobrotoxin-loaded poly （laetide-eo-glycolide） microspheres and their analgesic effect. Method The microspheres were prepared by modified water-in-oil-in-oil （w/o/o） emulsion solvent evaporation method. A tail flick assay was used to evaluate the analgesic activity of the microspheres after nasal administration. Result The microspheres with high entrapment efficiency （ 〉 80% ） and average diameter of about 25 um could be prepared by modified water-in-oil-in-oil （w/o/o） emulsion solvent evaporation method. A tail flick assy showed the evidence for their analgesic effect. Conclusion When Chinese cobraneurotoxin is loaded into microsphere and then the microsphere is administered through nasal eavity, its analgesic effect is enhanced significantly.

关 键 词：眼镜蛇神经毒素 PLGA 微球 镇痛 大鼠甩尾法 鼻 黏膜 

分 类 号：R-332[医药卫生] R452