翼状胬肉中血管内皮生长因子、内皮抑素的表达以及新生血管生成情况  

Expressions of vascular endothelial growth factor,endostatin and microvessel density in pterygia tissues

作　　者：彭畅[1] 蒋正轩[2] 梁坤[2] 陶黎明[2] 

机构地区：[1]安徽医科大学研究生学院,合肥230032 [2]安徽医科大学第二附属医院眼科,合肥230601

出　　处：《安徽医科大学学报》2014年第5期645-648,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金项目(编号:1208085MH178);安徽省高校自然科学基金重点项目(编号:KJ2013A147);安徽医科大学校基金(编号:2013xkj024)

摘　　要：目的通过研究翼状胬肉组织中新生血管数量、促进血管生成因子以及抑制血管生长因子的表达情况,探讨翼状胬肉组织中促进血管形成和抑制血管生成的情况。方法手术获得正常的结膜组织35例(对照组)及翼状胬肉组织40例(实验组),用免疫组化及Western blot的方法分别检测两组样本中新生血管密度(MVD)、血管内皮生长因子(VEGF)、内皮抑素(Endostatin)的表达情况。其中,CD31是标记组织MVD的指标。结果①免疫组化法结果显示:在翼状胬肉组织中MVD的含量明显高于正常结膜组织;VEGF在正常球结膜组织中仅有少量阳性表达,在翼状胬肉组织中阳性细胞明显增多,着色显著加深;翼状胬肉组织中上皮细胞基底细胞层中的Endostatin抗原阳性表达则明显少于正常球结膜组织。②Western blot结果显示人正常球结膜与翼状胬肉中VEGF及Endostatin蛋白表达存在差异。翼状胬肉组织中VEGF蛋白/β-actin(1.46±0.59)显著高于正常球结膜组/β-actin(0.46±0.45),差异有统计学意义(P<0.05);翼状胬肉组织中Endostatin蛋白/β-actin(0.58±0.11)显著低于正常球结膜组/β-actin(1.54±0.12),差异有统计学意义(P<0.05)。结论在翼状胬肉中发现其MVD及促血管生长因子的表达高于正常结膜组织,而血管抑制因子的表达含量降低,这一血管平衡的打破可能是翼状胬肉的发病基础之一。Objective To investigate the angiogenic imbalance in pterygium tissues of Chinese patients. Methods Forty pterygia and thirty-five normal bulbar conjunctivas were obtained. Proangiogenic and antiangiogenic factors such as vascular endothelial growth factor （VEGF） and endostatin were texted by immunohistochemical and western-blot. And microvessel density （MVD） was evaluated with immunoassaying for CD31. Results Our study revealed that the positive rate of VEGF significantly increased in the pterygium samples compared to the normal conjunctiva samples. Immunohistochemieal staining of pterygium tissues indicated less intense staining of endostatin in pterygium comparing normal bulbar conjunctivas. And microvessel density was higher in the pterygium tissues than normal conjunctiva. Conclusion The finding of high levels of proangiogenic factors and low levels of antiangiogenic factors in pterygia confirm that both proangiogenic and antiangiogenic factors are known to play an important role in human pterygium pathogenesis.

关 键 词：翼状胬肉 血管平衡 血管内皮生长因子 内皮抑素 

分 类 号：R777.33[医药卫生—眼科]