钙对化学诱抗剂诱导番茄叶片酚类物质含量的影响  

Effect of Calcium on Content of Phenolic Compounds Induced by Chemical Elicitors in Tomato Leaves

作　　者：余朝阁[1,2,3] 周娜 李天来[1] 刘志恒[2] 

机构地区：[1]沈阳农业大学园艺学院/设施园艺省部共建教育部重点实验室,沈阳110161 [2]沈阳农业大学植物保护学院,沈阳110161 [3]沈阳工学院,辽宁抚顺113122 [4]本溪市化学工业学校,辽宁本溪117000

出　　处：《沈阳农业大学学报》2014年第1期6-10,共5页Journal of Shenyang Agricultural University

基　　金：辽宁省教育厅项目(L2011115);国家"十二五"科技支撑计划项目(2011BAD12B03);辽宁省重大科技攻关项目(2011215003)

摘　　要：为探索钙对化学诱抗剂水杨酸(SA)、茉莉酸甲酯(MeJA)、β-氨基丁酸(BABA)和龙胆酸(GA)诱导番茄酚类物质积累的影响,在番茄6叶期用上述化学诱抗剂处理第3叶片,并分别喷施CaCl2、蒸馏水(H2O)、LaCl3和EGTA,然后测定各处理第3叶片(诱导叶)和第5叶片(非诱导叶)中酚类物质含量的变化。结果表明:(1)化学诱抗剂处理后,番茄诱导叶及其上位非诱导叶中酚类物质含量均升高;其中,SA、MeJA和GA处理后第2天,酚类物质含量迅速升高并达到高峰,而BABA处理后第1天酚类物质含量即达到高峰。(2)外源Ca2+显著促进诱抗剂对番茄叶片中酚类物质积累的诱导,4种诱抗剂与Ca2+共同处理,番茄叶片中酚类物质含量比相应诱抗剂单独处理高10%以上;而Ca2+螯合剂EGTA和质膜钙通道抑制剂LaCl3,则不同程度抑制这些诱抗剂诱导番茄叶片中酚类物质含量的提高。由此认为,钙对上述诱抗剂诱导番茄叶片中酚类物质的合成具有正调控作用。In order to investigate the effect of calcium on synthesis of phenolic compounds induced by salicylic acid （SA）, Methyl jasmonate （MeJA）, β-aminobutyric acid （BABA） and gentisic acid （GA） in tomato, the 3rd leaf was treated with above chemicals in six-leaf stage. At the sametime, these plants were sprayed with CaCl2, distilled water, LaCl3 or EGTA respectively. And then, content of phenolic compounds in the 3rd （induced） and 5th （un-induced） leaves were assayed at different time after treatments. Results showed that：（1） Content of phenolic compounds both in induced and un-induced leaves increased after treatment with chemical elicitors. It reached the peak on the 2nd day after treated with SA, MeJA and GA or on the 1st day after treated with BABA. （2） Content of phenolic compounds induced by chemical elicitors was promoted by exterior Ca2＋. Content of phenolic compounds in leaves treated with chemical elicitors and Ca2＋ was 10% above in leaves treated with chemical elicitors only. However, it was suppressed by calcium inhibitors such as Ca2＋ chelator EGTA and plasmalemma Ca2＋ channel blocker La3＋variously. Thus, we speculated that Ca2＋ play a positive regulation role in the synthesis of phenolic compounds induced by chemical elicitors in tomato leaves.

关 键 词：番茄 钙 化学诱抗剂 酚类物质 

分 类 号：S641.2[农业科学—蔬菜学]