响应面法优化耐盐烃降解菌培养基的初步研究  

Optimization of Medium Ingredients of Salt Tolerant Bacteria Hydrocarbon Degradation by Response Surface Methodology

作　　者：徐斌[1,2] 张祥胜[1,2] 

机构地区：[1]江苏省滩涂生物资源与环境保护重点建设实验室 [2]盐城师范学院生命科学与技术学院

出　　处：《油气田环境保护》2014年第2期7-8,11,共3页Environmental Protection of Oil & Gas Fields

基　　金：国家自然科学基金项目(No.10905035);江苏省滩涂生物资源与环境保护重点建设实验室开放课题(JLCBE11010)资助

摘　　要：海洋溢油污染对海洋和沿海滩涂生态环境造成严重威胁,筛选耐盐烃降解菌株和优化烃降解茵培养条件至关重要。以实验室筛选的一株烃降解菌为研究对象,在已有的单因素试验的基础上,采用响应面法对烃降解菌培养基进行优化,结果表明:当酵母粉质量浓度为2.74 g/L,NH1NO_3质量浓度为1.47 g/L,磷酸盐质量浓度为1.22 g/L时,烃降解率为65.81%,比单因素优化试验最优水平下的烃降解率增加14.58个百分点。The spilled crude oil pollution in ocean poses a heavy threat on ocean ecosystem and the coastal wetland,therefore,the isolation of salt resistant petroleum degrading microorganisms and the optimization of petroleum degrading media are crucial in dealing with this problem.With a strain isolated by the research group as the crude oil degrader,and based on former single factor experiments,hydrocarbon degradation medium was optimized by response surface methodology.When yeast powder content was 2.74g/L,NH_4NO_3 content was1.47 g/L and phosphate content was 1.22 g/L,the hydrocarbon degradation rate was 64.81%.Compared with optimized levels with single factor optimization experiments,the hydrocarbon degradation rate was increased by 14.58%.

关 键 词：产表面活性剂烃降解菌 响应面法 培养基优化 

分 类 号：X172[环境科学与工程—环境科学] X55