不同添加物及浓度对铁皮石斛组织培养的影响  

Effects of different additives and concentrations on the tissue culture of Dendrobium officinale

作　　者：罗焕明 邓瑞云 刘晓津 邱道寿 张蕾 蔡时可 

机构地区：[1]广东省农科院作物研究所/广东省农作物改良重点实验室,广东广州510640

出　　处：《广东农业科学》2014年第7期30-32,共3页Guangdong Agricultural Sciences

基　　金：广东省产学研项目(2011B090400537;2012B0911 00168);广东省社会发展重点项目(2011A030100002);广东省良种培育和引进项目(粤财农[2011]584号;粤农计[2011]143号)

摘　　要：以无菌播种取得的铁皮石斛无菌苗作为试验材料,研究1/2 MS培养基中添加不同浓度的添加物对铁皮石斛的增殖、生根的影响。结果表明:马铃薯汁和木瓜乳有利于诱导芽的增殖,其中又以1/2 MS+NAA 0.2 mg/L+蔗糖30 g/L+马铃薯200 g/L培养基最有利于诱导芽的增殖;香蕉乳有利于诱导生根,其中又以1/2 MS+NAA 0.5 mg/L+蔗糖30 g/L+香蕉100 g/L培养基最有利于诱导芽生根。The effects of different additives on proliferation and rooting culture of Dendrobium officinale were studied. The results showed that： potato and papaya were conductive to bud proliferation, among which the 1/2 MS＋NAA 0.2 mg/L＋ sucrose 30 g/L＋200 potato g/L medium was the best; banana promoted the rooting induction, in which 1/2 MS＋NAA 0.5 mg/L＋ sucrose 30 g/L＋Banana 100 g/L medium was the best to induce the rooting of the shoots.

关 键 词：铁皮石斛 组织培养 添加物 

分 类 号：S567.23[农业科学—中草药栽培]