老龄肾阳虚证体质差异表达基因筛选及半定量RT-PCR验证研究  

Study on the differentially expressed genes of aged kidney-yang deficiency syndrome constitution and verify genes with RT-PCR

作　　者：许嗣立[1] 李炜弘[1] 贾波[1] 崔珈铭[1] 史年刚[1] 

机构地区：[1]成都中医药大学,成都611137

出　　处：《中华中医药杂志》2014年第5期1568-1572,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.81173169);教育部科技重点项目(No.211158)~~

摘　　要：目的:筛选并验证与老龄肾阳虚证体质相关的差异表达基因。方法:采用SAM 3.1软件分析前期所获老龄肾阳虚差异基因表达谱,从差异表达基因谱中筛选出8个基因,并从差异表达基因功能、分子功能分类、分子通路等层面分析探讨老龄肾阳盛衰基因调控模式,通过调节Delta值,使得假阳性率为0,获得3个基因,选取10例肾阳虚证患者和10名正常对照者,采用半定量RT-PCR检测其血液中这3个基因的表达情况,验证与老龄肾阳虚证相关的这3条差异表达基因。结果:这3个基因在10例老龄肾阳虚证患者和10名正常对照者间的表达水平存在明显差异。结论:老龄肾阳虚证体质的相关基因涉及到衰老、肿瘤发生、生殖系统疾病等,集中体现了肾阳虚衰对本脏功能维持作用的减弱及脑肾关系。Objective： To filter and verify the relevance of the differentially expressed genes of aged kidney-yang deficiency syndrome constitution. Methods： From the earlier gene expression profile chip, 8 genes in the differentially expressed genes were filtered and the gene regulation mode of kidney-yang deficiency from differentially expressed genes were studied, gene functional classification and molecular pathway by SAM 3.1. After delta regulation and false positive rate of 0 control, 3 genes were filtered in the differentially expressed genes. 10 elderly patients with kidney-yang deficiency and 10 healthy people were selected, and 3 genes＇ expression were detected in the blood by the semi-quantitative skill of the RT-PCR, and the differentially expressed genes which correlated with the aged kidney-yang deficiency syndrome were verified. Results： There were 3 genes that had significant differences in expression between the 10 elderly patients with kidney-yang deficiency and 10 healthy people. Conclusion： The related genes of the aged kidney-yang deficiency syndrome was related to senility, tumor and reproductive system disease. It reflected that the function of kidney became weak from yang-deficiency and reflected the relationship between brain and kidney.

关 键 词：老龄肾阳虚证 差异表达基因 RT-PCR 衰老 

分 类 号：R229[医药卫生—中医基础理论]