Galectin-3基因多态性与乳腺癌相关性的研究  

The correlation of Galectin-3 gene polymorphism and breast cancer

作　　者：李慧兰[1] 李帅[1] 翟丽丽[1] 郭晓静[1] 付丽[1] 

机构地区：[1]天津医科大学肿瘤医院乳腺病理研究室,国家病理重点学科,国家肿瘤临床医学研究中心,天津市肿瘤防治重点实验室 乳腺癌教育部防治重点实验室,300060

出　　处：《中华内分泌外科杂志》2014年第2期105-108,共4页Chinese Journal of Endocrine Surgery

基　　金：国家自然科学基金项目（30930038）

摘　　要：目的：探讨Galectin-3（ lectin， galactoside-binding soluble ， LGALS3）基因-191位基因多态性（ rs4644）与中国女性乳腺癌发生的关系。方法应用限制性片段长度多态性聚合酶链反应（ reaction-re-striction fragment length polymorphism ， PCR-RFLP）方法检测172例乳腺癌患者肿瘤组织和17例良性乳腺组织rs4644基因型，随机选取（随机数字由计算机生成）10例PCR样本测序，验证PCR-RFLP的实验结果。结果乳腺癌患者中CC、CA及AA基因型的频率分别是72.7％、25.0％及2.3％，良性对照组中则分别为52.9％、41.2％及5.9％；乳腺癌患者中C、A等位基因的频率分别为85.2％、14.8％，良性对照组中则分别为73.5％、26.5％；二者基因型及等位基因的频率差异无统计学意义（ P＞0.05）；rs4644 A等位基因更容易出现在肿物 T ＞2 cm（χ2＝8.295，P ＝0.004）及 Ki67＜14％的乳腺癌人群（χ2＝3.917，P ＝0.048）。结论 Galectin-3基因191位基因多态性rs4644与乳腺癌的发生无明显相关性，其与肿瘤大小及Ki67密切相关。Objective To investigate the association of Galectin-3 （ lectin, galactoside-binding soluble , LGALS3）gene-191（rs4644）polymorphism and breast cancer in Chinese female population .Methods Galectin-3 gene rs4644 polymorphisms was genotyped by polymerase chain reaction-restriction fragment length polymor-phism（PCR-RFLP）in 172 breast cancer patients and 17 controls.The experimental results of PCR-RFLP were verified and sample of 10 cases were sequenced randomly .Results The frequency of CC ,CA and AA genotype in breast cancer group and normal control group was 72.7%, 25.0%, 2.3% and 52.9%, 41.2%, 5.9%, respectively .The frequency of C and A allele in breast cancer group and normal control group was 85.2%, 14.8%and 73.5%, 26.5% respectively .The genotype and frequency of allele had no statistical difference between breast cancer patients and the control group .rs4644C allele was more likely to occur in breast cancer patients with T＆gt;2 cm （χ2 =8.295,P=0.004） and Ki67＆lt;14%（χ2 =3.917,P=0.048）.Conclusions There is no significant association between Galectin-3 gene 191 polymorphism rs4644 and susceptibility of breast cancer in Chinese female population .It is closely associated with the size of tumor and Ki 67.

关 键 词：乳腺癌 GALECTIN-3 单核苷酸多态性 GALECTIN-3 

分 类 号：R737.9[医药卫生—肿瘤]