miR-430基因簇的生物信息学分析  

Bioinformatics Analysis of miR-430 Gene Cluster

作　　者：李小慧[1] 王凯[1] 吴建盛[1] 汤丽华[1] 

机构地区：[1]南京邮电大学地理与生物信息学院,江苏南京210023

出　　处：《南京邮电大学学报（自然科学版）》2014年第2期122-126,132,共6页Journal of Nanjing University of Posts and Telecommunications：Natural Science Edition

基　　金：南京邮电大学引进人才科研启动基金(NY211031);南京邮电大学实验室工作研究课题(201310)资助项目

摘　　要：很多microRNA(miRNA)基因在基因组上紧密排列形成miRNA基因簇,mir-430是目前已发现的最大miRNA基因簇,但其起源和进化方面却是未知的。为了揭示mir-430基因簇的起源及其在相关物种的进化关系,文中基于miRNA序列同源保守的特点,采用BLAST程序在NCBI基因数据库中搜索mir-430序列,在14个物种中共搜索到35个mir-430基因序列,这14个物种都为硬骨鱼类。多序列对比发现mir-430基因簇成熟序列的第2到第8位碱基以及第16到第22位碱基为保守序列。进化分析表明七鳃鳗的pma-mir-430基因可能是此基因家族最早出现的基因形式,并且pma-mir-430e可能是其他物种mir-430基因的祖先基因。祖先基因经过个别碱基的缺失及突变、串联重复和大片段重复等方式形成了mir-430基因簇。该研究通过分析不同物种中mir-430基因簇的特征,揭示mir-430基因簇的分子进化规律,为其调控网络和功能研究提供理论基础。Many of miRNA genes are closely formed as clusters in the genome. It is found that miR-430 is the largest miRNA gene cluster; however, little is known about its origin and evolution. In order to reveal the evolutionary origin of miR-430 gene cluster, homologous studies are carried out to search for novel miR-430 genes by BLAST programme in this report. The 35 miR-430 genes from 14 species are found to be teleost. The result from multiple sequence alignment of mature miR-430 gene sequences shows that the second to eighth bases and the sixteenth to twenty-twoth bases are conservative. Phylogenetic analysis re- veals that miR-430 gene of Petromyzon marinus is the earlist forms in this family, and pma-miR-430e is the ancestral gene of miR-430 in other species, miR-430 genes are generated after deletion and mutation of individual bases, tandem duplication and large segment duplication in ancestral genes. The study re- veals the miR-430 gene cluster molecular evolution by analyzing characteristics of different miR-430 gene clusters, thus it provides the theoretical basis on their regulation and function in cell network.

关 键 词：mir-430 基因簇 生物信息学预测 分子进化 miR-430 

分 类 号：Q349.5[生物学—遗传学] Q75