hMMS2siRNA联合抗癌药对结肠癌细胞SW480增殖与凋亡的影响  

hMMS2 siRNA combined with anticancer drugs on influence of proliferation and apoptosis of colon cancer cells

作　　者：王婷[1] 隋御[2] 张蕾[1] 李元杰[2] 贾伟[3] 金彩霞[4] 徐方[2] 

机构地区：[1]宁夏医科大学检验学院宁夏回族自治区生殖与遗传重点实验室,银川750004 [2]宁夏医科大学遗传学与细胞生物学系,银川750004 [3]宁夏医科大学总医院医学实验中心,银川750004 [4]同济大学第十附属医院转化医学研究中心,上海200072

出　　处：《中国免疫学杂志》2014年第4期480-485,共6页Chinese Journal of Immunology

基　　金：国家自然科学基金项目资助(No.31360251&No.81060170)

摘　　要：目的:探讨hMMS2 siRNA联合奥沙利铂和槐定碱对结肠癌细胞增殖和凋亡的影响。方法:运用RNAi技术降低结肠癌细胞系SW480中hMMS2表达,用实时荧光定量PCR和免疫荧光化学检测hMMS2表达量,选择hMMS2低表达具有统计学意义的SW480细胞作为实验组,不作任何处理的SW480细胞作为空白对照组,转染阴性质粒的SW480细胞作为阴性对照组。将三组细胞加入奥沙利铂和槐定碱培养,48 h后运用MTT实验及流式细胞术对各组细胞进行增殖和凋亡检测。结果:与空白对照组相比,hMMS2 siRNA、奥沙利铂、槐定碱单独作用均能促进SW480细胞凋亡并抑制其增殖;与药物空白组相比,hMMS2 siRNA联合一种抗癌药物(奥沙利铂或槐定碱),对SW480细胞抑制增殖和促进凋亡作用比单一因素明显;hMMS2siRNA、奥沙利铂、槐定碱三种因素联合比任两种联合作用效果更加显著,以上结果均具有统计学意义(P<0.05)。结论:hMMS2 siRNA、奥沙利铂、槐定碱均能抑制结肠癌细胞系SW480增殖和促进其凋亡,三者联合具有协同作用。Objective ：Explore ablation of hMMS2 significantly reduced joint anticancer drugs （oxaliplatin and sophoridine） tract the influence of proliferation and apoptosis of colon cancer cells. Methods： We experimentally suppressed the hMMS2 expression in human colon cancer cells by the interference RNA technology （RNAi） as monitored by Real-time Q-PCR and immunocytochemistry. Compared with untreated or mock-treated cells, SW480 cells that express rate significantly reduced were elected as case group, While set the negative control group and the blank control group. Oxaliplatin and sophoridine were added to each group of cells for 48h respec- tively. MTF assay and flow cytometry were used for detection of cells proliferation and apoptosis in each group. Results： Compared with the blank control group, ablation of hMMS2 significantly reduced, or the anti-cancer drug （ oxaliplatin or sopboridine） alone could promote the apoptosis and inhibit the proliferation of SW480 cells ; compared with the drug blank group, ablation of hMMS2 significant- ly reduced combined with one anti-cancer drugs （oxaliplatin or sophoridine） on the inhibition of the proliferation and promotion of the apoptosis of SW480 cells was significantly higher than a single factor; Ablation of hMMS2 significantly reduced combined with oxalipla- tin and sophoridine was more significant than the effect of any two combined factors. Above results were all statistically significant （ P 〈 0.05）. Conclusion： Ablation of hMMS2 significantly reduced, oxaliplatin and sophoridine can inhibit the proliferation and promote the apoptosis of SW480 cells,the combination of the three has a synergistic effect.

关 键 词：hMMS2 结肠癌 流式细胞术 

分 类 号：R735.35[医药卫生—肿瘤]