响应面法优化蔗梢多糖超声波提取工艺  

Optimization on ultrasonic extraction of polysacchrides from sugarcane(Saccharum officinarum L.) tops by response surface analysis

作　　者：何雪梅[1,2,3] 孙健[1,4] 李丽[1,2] 李昌宝[1,2] 叶文才[3] 董怡[4] 

机构地区：[1]广西农业科学院农产品加工研究所 [2]广西作物遗传改良生物技术重点试验室,南宁530007 [3]暨南大学药学院中药及天然药物研究所,广州510632 [4]华南理工大学轻工与食品学院,广州510640

出　　处：《南方农业学报》2014年第3期458-462,共5页Journal of Southern Agriculture

基　　金：农业部公益性行业(农业)科研专项项目(201203072-03);国家级引进国外技术;管理人才项目(20134500069);广西留学人员科技活动项目择优资助项目(桂人社办发[2012]250号);广西农业科学院基本科研业务专项项目(桂农科2013YM03);广西重点试验室建设项目(12-071-09)

摘　　要：【目的】优化蔗梢多糖的提取工艺条件,为蔗梢多糖的开发利用提供技术参考。【方法】以多糖提取率和DPPH自由基清除率为指标,采用响应面法分析优化超声波提取蔗梢多糖的工艺条件。【结果】超声波提取蔗梢多糖最佳工艺为:超声功率640 W,提取温度69℃,提取时间29 min,在此条件下,其多糖提取率为4.68%,DPPH自由基清除率为74.89%。【结论】采用响应面法优化蔗梢多糖超声波提取工艺具有较高的可行性。[Objective]The extraction parameters of polysaccharides in sugarcane （Saccharum officinarum L.） tops were optimized to provide references for utilizing sugarcane top polysaccharides. [Method]With polysaccharides yield and DPPH scavenging activity as the indicators, response surface analysis （RSA） was used to optimize the ultrasonic extraction conditions for polysaccharides from sugarcane tops. [Result]The optimal conditions were ultrasonic power of 640 W, ex-traction temperature of 69 ℃, and extraction time of 29 min, under which the extraction yield and the DPPH scavenging rate of polysaccharides was 4.68% and 74.89%, respectively. [Conclusion]The response surface methodology was a rea-sonable method to optimize the extraction parameters of sugarcane top polysaccharides.

关 键 词：蔗梢 多糖 超声波提取 提取率 DPPH自由基清除率 响应面法 

分 类 号：TQ281[化学工程—有机化工]