微生物来源抗肿瘤抗生素SIPI-228的菌种选育及发酵工艺优化  

Improvement of Fermentation Technology and Strain Breeding for Anti-cancer Antibiotic SIPI-228

作　　者：熊磊[1] 高苹[1] 周斌[1] 胡海峰[1] 

机构地区：[1]中国医药工业研究总院上海医药工业研究院,创新药物与制药工艺国家重点实验室,上海200040

出　　处：《中国医药工业杂志》2014年第5期417-420,共4页Chinese Journal of Pharmaceuticals

基　　金：科技部"十二五"重大仪器专项(2011YQ15004808);国家"十二五""重大新药创制"科技重大专项(2012ZX09301002-003)

摘　　要：化合物SIPI-228是一种具有抗肿瘤、抗真菌活性的环肽类抗生素。以本室保藏的紫色色杆菌Chromobacterium violaceum SIPI-820作为出发菌,通过紫外(UV)以及UV与硫酸二乙酯联合诱变,获得一株高产突变株SIPI-LH7-16,其发酵产物SIPI-228的产量较出发菌株提高了354%。通过优化摇瓶发酵工艺,SIPI-228的产量达到531 mg/L,较初始工艺提高了254%;5 L发酵罐的发酵水平达到810 mg/L。SIPI-228 is a cyclic peptide antibiotic with anticancer and antifungal activities. In this paper, a Chromobacterium violaceum named as SIPI-820 kept in our lab. was used as the starting strain. By UV mutation and UV combined with diethyl sulfate treatment, a mutant named as SIPI-LHT-16 was selected and its productivity of SIPI-228 was 354% higher than that of the original strain. After improving its fermentation process, the yield of SIPI-228 in the optimal conditions of shake flask reached to 531 mg/L, which was 254% higher than the initial process. The yield of SIPI-228 reached to 810 mg/L in a 5 L fermentor.

关 键 词：环肽类抗生素 紫色色杆菌 SIPI-228 菌株选育 

分 类 号：TQ465[化学工程—制药化工]