SHP-1和JAK3基因在成人急性髓系白血病患者中的表达  

Expressions of SHP-1 and JAK3 genes in acute myloid leukemia patients

作　　者：杨生梅[1] 周永安[1] 郝国平[1] 王晓欢[1] 武坚锐[1] 杨光[1] 

机构地区：[1]山西省儿童医院,山西太原030013

出　　处：《临床医药实践》2014年第5期355-356,374,共3页Proceeding of Clinical Medicine

摘　　要：目的:分析SHP-1基因和JAK家族基因在成人急性髓系白血病(AML)患者中的表达及相关性,以探讨两者在AML发病机制中的作用。方法:选取初发急性髓系白血病患者93例及健康志愿者或完全缓解(CR)患者20例为研究对象,分别应用荧光定量聚合酶链(FQ-PCR)和反转录聚合酶链反应(RT-PCR)方法检测骨髓单个核细胞中SHP-1和JAK3基因的mRNA表达情况,并分析两者的相关性及其与预后的关系。结果:SHP-1在AML患者中的表达水平明显低于完全缓解组和健康对照组(P<0.01),而JAK3表达水平明显高于完全缓解组和健康对照组(P<0.05);SHP-1和JAK3在mRNA水平方面呈现负相关(P<0.05)。结论:SHP-1可能是白血病的抑癌基因,AML细胞中SHP-1与JAK3的mRNA表达呈负相关,这种负调控作用的机制有待进一步研究。Objective：To investigate the expression of SHP - 1 and JAK3 mRNA in the primary acute myloid leukemia （AML） patients and their impact on leukemogenesis. Methods：The expression of SHP - 1 and JAK3 mRNA in 93 AML pa- tients and 20 healthy or complete remission（CR） adults as normal controls were measured with fluoreseenee quantitative poly- merase chain reaction （FQ -PCR） and reverse transcription polymerase chain reaction（ RT -PCR）. The relationship between the expressions of SHP - 1 and JAK3 was analysed. Results ： The expression of SHP - 1 mRNA in de novo AML patients was significantly lower than that in complete remission and healthy control group（ P 〈 0.01 ）. The expressions of JAK3 in AML were significant higher than complete remission and healthy control group （ P 〈 0.05 ）. There was a negative correlation between SHP - 1 and JAK3 mRNA （ P 〈 0.05 ）. Conclusion ： SHP - 1 gene is a antioncogene. There was a negative correlation between SHP - 1 and JAK3 mRNA. Such negative regulation mechanism needs further study.

关 键 词：白血病 髓系 SHP-1 JA硒 

分 类 号：R733.71[医药卫生—肿瘤]