带捕集阱的自动化静态顶空测定苹果酯类香气物质  

Volatile Esters in Apple by Trap and Automated Static Headspace

作　　者：史作安[1] 王超[1] 颜卫东[1] 杨超[1] 

机构地区：[1]山东农业大学农业生物学国家重点实验室,农业生物学国家级实验教学示范中心,山东泰安271018

出　　处：《实验室研究与探索》2014年第3期25-28,共4页Research and Exploration In Laboratory

基　　金：农业生物学国家级实验教学示范中心建设项目(教高函[2007]21号);山东省高等教育教学改革研究课题(2012326)

摘　　要：采用带捕集阱的自动化静态顶空和气相色谱质谱联用法对苹果酯类香气成分进行定性、定量分析,利用控制单因素变量法对顶空萃取条件的优化,确定了最佳分离测定条件为:捕集肼温度45℃,平衡时间40 min,色谱柱Rtx-1 MS(30 m×0.25 mm×0.25μm)。结果显示,从成熟的富士苹果中分离鉴定出35种酯类香气成分,结合计算机检索和峰面积归一法对各组分进行定性定量分析,获得相对质量分数较高的成分分别为丁酸乙酯(0.47%)、2-甲基-丁酸乙酯(0.22%)、乙酸丁酯(5.85%)、2-甲基乙酸丁酯(27.21%)、丁酸丙酯(1.81%)、丙酸丁酯(2.32%)、乙酸戊酯(1.8%)、2-甲基-1-丁酸异丙酯(3%)、丁酸丁酯(2.92%)、己酸乙酯(2%)、乙酸己酯(17.21%)、2-己烯-1-醇乙酸酯(3.85%)、2-甲基丁酸甲酯(5.23%)、丁酸己酯(4%)和2-甲基-丁酸己酯(8.1%)。本方法准确、迅速、节约、环保,对园艺产品品质分析有一定的借鉴作用。The volatile compounds in apple was analyzed by headspace gas instrument with adsorption trap,and was sampled and injected to gas chromatography. The parameters of headspace trap included equilibrium temperature and time which were optimized by single factor variable method. Results showed that the optimal values were equilibrium temperature 45 ℃,equilibrium time 40 min with Rtx-5MS（ 30 m × 0. 32 mm × 0. 25 μm） column. 35 volatile ester components were identified in ripening Fuji apple by the method presented. The major components quantitatively were calculated by compound library screening and area normalization method. They were： butanoic acid,ethyl ester（ 0. 47%）,butanoic acid,2-methyl-,ethyl ester（ 0. 22%）,acetic acid,butyl ester（ 5. 85%）,1-butanol,2-methyl-, acetate（ 27. 21%）,butanoic acid,propyl ester（ 1. 81%）,propanoic acid,butyl ester（ 2. 32%）,acetic acid,pentyl ester（ 1. 8%）,butanoic acid,2-methyl-,1-methylethyl ester（ 3%）,butanoic acid,butyl ester（ 2. 92%）,hexanoic acid,ethyl ester（ 2%）,acetic acid,hexyl ester（ 17. 21%）,2-hexen-1-ol,acetate（ 3. 85%）,butyl 2-methyl butanoate（ 5. 23%）,butanoic acid,hexyl ester（ 4%） and butanoic acid,2-methyl-,hexyl ester（ 8. 1%）. The method is simple and fast and will benefit the quality analysis of horticultural products.

关 键 词：带捕集阱自动化静态顶空 气相色谱质谱联用 苹果 酯类香气 

分 类 号：S661.1[农业科学—果树学]