硫氰化钾与DCIC反应法测定烟草总植物碱  

Determination of Total Alkaloid in Tobacco by Reaction of Potassium Thiocyanate and Sodium Dichloroisocyanurate Dihydrate

作　　者：张杰[1] 马雁军[1] 石睿[1] 张威[2] 马莉[1] 王颖[2] 周骏[1] 

机构地区：[1]上海烟草集团有限责任公司技术中心北京工作站,北京市通州区万盛南街99号101121 [2]国家烟草质量监督检验中心

出　　处：《烟草科技》2014年第5期60-63,共4页Tobacco Science & Technology

摘　　要：为了准确测定烟草中总植物碱，降低检测过程中的安全风险，采用硫氰化钾和二水合二氯异氰尿酸钠（DCIC）为在线反应试剂，if,4用连续流动分析仪，建立了一种测定烟草中总植物碱的方法。通过优化反应条件，确定了硫氰化钾和DCIC的最佳反应浓度分别为0．124和0．04mol／L。运用此方法检测烤烟、白肋烟和香料烟等9种烟草样品，并将测定结果与现行行业标准方法的结果进行比较。结果表明：总植物碱的检出限为0．0018mg／mL，定量限为0．0062mg／mL，回收率为94．4％～105．4％。该方法避免使用剧毒的氰化钾试剂，降低了安全风险，适用于烟草中总植物碱的测定。To determine the total alkaloid in tobacco accurately and reduce the safety risk, a method was developed by using potassium thiocyanate and sodium dichloroisocyanurate dihydrate （DCIC） as reaction reagents in continuous flow analyzer. The preferable concentrations of potassium thiocyanate and DCIC were 0.124 and 0.04 mol/L, respectively. This method was compared with the current industrial standard method, the total alkaloid in 9 tobacco samples including flue-cured, burley and oriental tobacco were analyzed. The developed method featured that： the limit of detection and limit of quantification were 0.0018 and 0.0062 mg/mL, respectively; and the recoveries of the method ranged from 94.4% to 105.4%. This method is safer and suitable for the determination of total alkaloid in tobacco with the advantages of using no toxic potassium cyanate.

关 键 词：烟草 总植物碱 连续流动法 硫氰化钾 二水合二氯异氰尿酸钠(DCIC) 水萃取 

分 类 号：TS411.1[农业科学—烟草工业]