大肠癌中磷酸化丝氨酸/苏氨酸激酶和磷酸酶-张力蛋白同源体的表达及其临床意义  

Expression and clinical significance of phosphorylated AKT and phosphatase and tensin homolog in colorectal carcinoma

作　　者：凌华晃[1] 李涛[1] 罗爱华[2] 高媚[3] 李飞红[3] 姜汉国[3] 

机构地区：[1]广东医学院附属高州市人民医院肿瘤科, 525200 [2]广东医学院附属高州市人民医院病理科, 525200 [3] 广东医学院病理教研室

出　　处：《肿瘤研究与临床》2014年第4期253-256,共4页Cancer Research and Clinic

摘　　要：目的 检测大肠腺癌组织磷酸化丝氨酸/苏氨酸激酶（pAKT）、第10号染色体同源缺失性磷酸酶-张力蛋白同源体（PTEN）表达及其临床意义.方法 应用免疫组织化学SP法检测112例大肠癌pAKT和PTEN蛋白的表达.结果 大肠癌pAKT和PTEN的阳性表达率分别为79.5％（89/112）和47.3 ％（53/112）,与在大肠癌旁组织和腺瘤组织pAKT和PTEN的阳性表达率差异有统计学意义（P＜0.01）.pAKT的阳性表达与大肠癌肿瘤浸润深度、临床分期和淋巴结转移密切相关,差异均有统计学意义（均P＜0.05）.PTEN的阳性表达同肿瘤浸润深度、肿瘤组织分化程度、临床分期和淋巴结转移密切相关,差异均有统计学意义（均P＜ 0.05）.pAKT蛋白表达与PTEN蛋白表达呈负相关（P＜0.01）.结论 pAKT和PTEN与大肠癌发生、发展和转移密切相关,提示阻断PTEN-磷脂酰肌醇3激酶（PI3K）-AKT信号转导通路可能对大肠癌的治疗提供新的靶点.Objective To study the expression and clinical significance of phosphorylated AKT （pAKT） and phosphatase and tensin homolog （PTEN） in colorectal carcinoma tissues.Methods The expression of pAKT and PTEN were detected by immunohistochemical SP method in 112 case of colorectal carcinomas tissue.Results The positive rates of pAKT and PTEN expression were 79.5 % （89/112） and 47.3 % （53/112） in colorectal cancerous tissues,respectively,which showed a statistically significance when compared with those in adjacent normal and adenoma tissues.The positive rate of pAKT expression was closely related with the invasive depth,clinical staging and lymph node metastasis of colorectal carcinomas.The positive expression of PTEN was also closely related with the invasive depth,degree of differentiation,clinical staging and lymph node metastasis of colorectal carcinomas.The expressions of pAKT and PTEN were negative correlaed.Conclusion The results showed that the expression of pAKT and PTEN were closely related with progression and metastasis of colorectal carcinomas,which may provide a new therapeutic target for colorectal carcinomas by blocking PTEN/PI3K/AKT signaling pathway.

关 键 词：大肠肿瘤 磷酸化丝氨酸 苏氨酸激酶 第10号染色体同源缺失性磷酸酶-张力蛋白 

分 类 号：R735.34[医药卫生—肿瘤]