不同产地与采收期环草石斛的红外光谱研究  

Study on Dendrobium Loddigesii Rolfes from Different Regions and Harvest Periods by FTIR

作　　者：刘文杰[1] 孙志蓉[1] 许长华[2] 孙素琴[2] 魏鑫鑫[1] 陈龙[1] 杜远[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]清华大学化学系生命有机磷化学及化学生物学教育部重点实验室,北京100084

出　　处：《光谱学与光谱分析》2014年第5期1217-1221,共5页Spectroscopy and Spectral Analysis

基　　金：国家"十一五"科技支撑计划项目(2009BAI74B02-3);国家自然科学基金项目(30870262)资助

摘　　要：环草石斛 Dendrobium loddigesii Rolfe是中药石斛的源植物之一，以茎入药，具有滋阴清热、益胃生津的功效。本文采用红外光谱法对不同产地、不同采收期的环草石斛分别进行分析比较。结果显示，不同产地环草石斛红外光谱中，1035，1051，1078，1156，1500，1511，1736 cm -1等附近峰的相对峰强皆有显著差异，表明不同产地环草石斛的多糖、酮、酯类等成分的组成和含量有明显差异。在二维相关红外光谱中，越南、云南、广西、贵州的环草石斛分别有7，8，8，9个自动峰，且广西环草石斛的最强峰在1220 cm -1附近，不同于其他三个产地样品（980 cm -1）。不同采收期环草石斛的红外光谱中，1034～1023 cm -1主强峰的波数，（1617±177;4）cm -1次强峰的波数以及1078（1076，1079）cm-1峰的有无，皆随季节呈现出明显的阶段性变化，显示了环草石斛生长过程中所含多糖、酮类等成分积累的阶段性变化规律。应用红外光谱法能较好把握环草石斛的生长状况，帮助全面了解其成分组成与含量变化，对环草石斛的合理开发与高效利用有重要意义。Infrared Spectroscopy （IR） integrated with two dimensional correlation infrared spectroscopy （2DCOS IR） was em-ployed to rapidly discriminate Dendrobium loddigesii Rolfes （DR） from different regions and harvesting periods .The results showed that the IR peaks around 1 035 ,1 051 ,1 078 ,1 156 ,1 500 ,1 511 and 1 736 cm -1 had perceptible differences among DRs from different regions ,indicating that different DRs containing remarkable different compositions and contents of polysac-charides ,ketones and esters .2DCOS IR spectra of DRs from Vietnam ,Yunnan ,Guangxi ,Guizhou each had seven ,eight , eight ,nine auto peaks ,respectively ;furthermore ,DRs from Guagnxi had the strongest peak in 1 220 cm -1 ,which was distin-guish to those of other DRs （980 cm-1 ） .In the IR spectra of DRs from different harvest seasons ,the wave number of key peaks in （1 034-1 023）cm-1 ,the wave number of minor peaks in （1 617 ±177; 4）cm -1 ,as well as the presence of peaks in 1 078（1 076 , 1 079）cm -1 ,showed obvious periodic changes with the seasons ,which indicated the accumulation of polysaccharides and ketones from DRs displayed an evident periodic variability discipline .The application of FTIR in DRs could facilitate acquiring their growth conditions ,composition and content changes ,which would be significant in rational exploitations and utilizations of DR .

关 键 词：环草石斛 红外光谱 二阶导数光谱 二维相关红外光谱 

分 类 号：R917[医药卫生—药物分析学]