TPH-2基因rs4570625和G1463A交互作用与单相抑郁症关系  

Association of interaction of tryptophan hydroxylase isoform( TPH-2 ) genes with uniplor deression

作　　者：杨秀贤[1] 乔正学[1] 邱晓惠[1] 王琳[1] 马靖松[1] 焦阳[1] 王鹏[1] 杨艳杰[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院医学心理学教研室,黑龙江哈尔滨150081

出　　处：《中国公共卫生》2014年第5期560-562,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(81202213;31271093);教育部博士点基金(20102307110020)

摘　　要：目的探讨色氨酸羟化酶2(TPH-2)基因rs4570625和G1463A的交互作用与单相抑郁症的关系。方法提取中国北方地区300例单相抑郁症患者(病例组)和300名健康对照者(对照组)基因组DNA,采用PCR技术分别扩增包含TPH-2基因rs4570625和G1463A位点的基因组DNA片段,将PCR产物直接测序检测病例组及对照组的基因型,应用非条件logistic回归模型分析rs4570625和G1463A基因的交互作用。结果病例组与对照组单核苷酸多态性基因型分布符合Hardy-Weinberg平衡定律(P>0.05);单相抑郁症组与健康对照组TPH-2基因rs4570625位点基因型及等位基因频率比较,差异无统计学意义;G1463A基因型及等位基因频率2组比较差异均有统计学意义(均P<0.01),病例组携带的基因型G/G、A/G、A/A频率分别为6.0%、18.3%、75.7%,对照组分别为57.3%、2%、40.7%;交互作用分析显示,rs4570625 TG基因型与G1463A基因型对单相抑郁症患病具有正交互作用(ORAB=5.539,ORA=0.747,ORB=0.024,ORI>1)。结论 rs4570625与G1463A变异之间的交互作用在单相抑郁症发病机制中具有重要意义。Objective To explore the association of the interaction between tryptophan hydroxylase isoform（TPH-2） gene rs4570625 and G1463A with uniplor deression. Methods We collected specimens of peripheral blood from 300 uniplor depression patients and 300 healthy controls in a mental hospital in Harbin city. PCR was used to detect the poly- morphisms of rs4570625 and G1463A, and logistic regression model was used to analyze the interaction of rs4570625 and G1463A. Results The genotypic frequences of the single nucleotide polymorphism（SNP） did not deviant from Hardy- Weinberg equilibrium in both case and control groups （ P 〉 0.05 ）. Compared with the control group, no significant differ- ence in genotypes and alleles of TPH2 gene rs4570625 was found in patient group （ P 〉 0. 05 ）. There were significant differences in G/G,A/G, and A/A frequencies of TPH2 gene G1463A between the patient group（6. 0%, 18.3%, and 75.7% ）and control group （57. 3 %, 2%, and 40. 7 % ）. There was a positive interaction between rs4570625 and G1463A in uniplor depression patients （odds ratio E OR] AB = 5. 539, ORa = 0. 747, OR8 = 0. 024, OR1 〉 1 ）. Conclusion The inter- action between rs4570625 and G1463A plays an important role in the incidence of uniplor depression.

关 键 词：色氨酸羟化酶2(TPH-2) 交互作用 抑郁症 

分 类 号：R749[医药卫生—神经病学与精神病学]