P物质对高糖孵育心肌细胞缺氧／复氧损伤的影响  

Effect of Substance P on Anoxia-reoxygenation Induced Injury in Neonatal rat Cardiomyocytes Incubated in High Glucose Medium

作　　者：赵鑫[1] 陈璐[1] 郭政[1] 

机构地区：[1]山西医科大学第二医院麻醉科,山西太原030001

出　　处：《麻醉与监护论坛》2014年第1期54-57,共4页Forum of Anesthesia and Monitoring

摘　　要：目的：探讨P物质预处理对高糖孵育大鼠心肌细胞缺氧／复氧损伤的影响。方法：分离新生SD大乳鼠心脏为细胞悬液接种于细胞培养板，分别用正常糖和高糖培养基进行孵育，72h后将细胞随机分为5组，分别为正常对照组，高糖对照组，另外三组进行缺氧／复氧（A／R）处理：高糖A／R组，高糖SP＋A／R组和高糖SP＋D—SP＋A／R组。复氧后分别测定细胞凋亡率、缺氧液和复氧液中caspase-3活性以及LDH释放量。结果：高糖和缺氧／复氧均可引起细胞凋亡率增高，缺氧液和复氧液中CasPase-3活性以及LDH释放量均显著升高：SP可降低上述各项指标，且该作用可被NK-1受体拮抗剂（D-SP）逆转。结论：高糖孵育可造成心肌细胞损伤，缺氧／复氧可加剧高糖孵育的大鼠心肌细胞损伤．SP预处理可显著减轻该损伤，且该作用可能由NK-1受体介导。Objective. The aim of the study was to investigate the cardioprotective effect of substance P （SP） on anoxia/ reoxygenation （A/R） induced injury in cultured cardiomyocytes incubated in high glucose medium of neonatal rats. Methods. Cardiomyocytes were obtained from 1-3 day old Sprague-Dawley （SD） rats and were seeded in 6-well plates （3x105 cells/ml） and then incubate with normal glucose （5.5 mmol/L） medium or high glucose （25 mmol/L） medium respectively for 72 hours. Fifteen wells of the cultured cells were assigned to five groups （3 wells in each group, n=3） randomly： （1） the normal control group （incubated in normal glucose medium ,without any treatment of agent or A/R）, （2） the high glucose control group （incubated in high glucose medium ,without any treatment of agent or A/R）. The cells in following three groups experienced anoxia/reoxygenation （anoxia for 3 hours and then followed by reoxygenation for 2 hours）： （3） the high glucose plus A/R group （incubated in high glucose medium and experienced the anoxia/reoxygenation without exposing to any drug）, （4） the high glucose plus SP＋A/R group （incubated in high glucose medium, pretreated with 10-7mol/L of SP for 1 hour and then went through the anoxia/reoxygenation treatment） and （5） the high glucose plus SP＋D-SP＋A/R group （incubated in high glucose medium, exposed to lO-7mol/L of SP plus 10-6mol/L of D-SP （a specific antagonist of neurokinin-1 receptor） for 1 hour and then went through the anoxia/reoxygenation treatment）. In each group, apoptosis ratio of the cells, caspase-3 activity and concentration of lactate dehydrogenase （LDH） in culture media before and after reoxygenation were examined by TUNEL assay, ELISA and the detecting kit respectively. Results. The apoptosis ratio of cardiomyocytes, caspase-3 activity and LDH were all significantly increased in high glucose control group （p〈O.01）, compared with the normal control group. When compared with high gluco

关 键 词：P物质 高糖 心肌细胞 缺氧 复氧 

分 类 号：R[医药卫生]