紫外线抵抗相关基因在二烯丙基二硫诱导人白血病K562细胞自噬中的作用  

Value of Ultraviolet Radiation Resistance-associated Gene in the Diallyl Disulfide Induced Autophagy of Leukaemia K562 Cells

作　　者：王萍[1] 殷小成[1] 彭艳辉[1] 胡贤娇 杨云华[1] 

机构地区：[1]南华大学附属第一医院儿科,湖南衡阳421001

出　　处：《肿瘤基础与临床》2014年第2期93-96,共4页journal of basic and clinical oncology

基　　金：国家自然科学基金资助项目(编号:31271482)

摘　　要：目的观察紫外线抵抗相关基因(UVRAG)在二烯丙基二硫(DADS)诱导白血病K562细胞自噬中的作用。方法以K562细胞为细胞模型,利用透射电镜观察细胞的超微结构自噬体的形成;采用吖啶橙染色和荧光显微镜观察酸性囊泡的形成以检测DADS诱导K562细胞自噬;RT-PCR检测自噬相关基因UVRAG mRNA的表达水平。结果透射电镜显示:与空白组和溶媒组比较,DADS(40 mg·L-1)处理组可观察到胞质内出现大量自噬体。吖啶橙染色荧光显微镜下可见到各DADS处理组K562细胞胞浆中的酸性囊泡较空白组和溶媒组明显增多。RT-PCR结果显示,DADS作用K562细胞12 h后,各DADS处理组UVRAG mRNA表达水平均高于空白组和溶媒组。结论 DADS能诱导K562细胞自噬,其机制可能与上调UVRAG mRNA表达有关。Objective To observe the value of ultraviolet radiation resistance-associated gene（UVRAG）in the diallyl disulfide（DADS）induced autophagy of K562 cells. Methods K562 cells were chosen for this study. Their ultrastructure was observed by transmission election microscopy. The formation of autophagic vacuoles were observed by fluorescence microscope after acridine orange staining. RT-PCR was used to detect the expression levels of UVRAG mRNA. Results Compared with the blank group and the menstruum group,large amount of autophago-somes were observed in the cytoplasm of K562 cells treated with DADS（40 mg＆#183;L - 1 ）;amount of autophagic vacu-oles observed in all the DADS treatment groups were observed;the UVRAG mRNA expression levels were higher in all the DADS treatment groups after DADS treatment for 12 h（P ＆lt; 0. 05）. Conclusion DADS can induce autoph-agy of K562 cells,it may be related to the up-regulation of UVRAG mRNA expression.

关 键 词：二烯丙基二硫 白血病 K562细胞 自噬 紫外线抵抗相关基因 

分 类 号：R733.7[医药卫生—肿瘤] R730.23[医药卫生—临床医学]