番茄红素对离体豚鼠心脏缺血/再灌注损伤的影响  

Effects of Lycopene on Ischemia/Reperfusion Injure in Isolated Heart of Guinea Pig

作　　者：张建武[1] 朱姝[2] 刘文虎[1] 李生茂[1] 余娴[1] 闵冬雨[3] 

机构地区：[1]川北医学院药理教研室,四川南充637100 [2]中国医科大学口腔医院,沈阳110002 [3]辽宁中医药大学附属医院,沈阳110032

出　　处：《中国实验方剂学杂志》2014年第10期134-137,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81102288)

摘　　要：目的:探讨番茄红素对豚鼠离体心脏缺血/再灌注损伤的影响。方法:将30只豚鼠离体心脏随机分为5组:正常组、缺血/再灌注组(Model)、番茄红素低剂量组(2.5 mg·L-1)、番茄红素中剂量组(5 mg·L-1)、番茄红素高剂量组(10 mg·L-1),模型组全心缺血40 min之后,K-H液继续灌注60 min,治疗组的灌流液中加入不同质量浓度的番茄红素。观察的指标有:心率、冠脉流量、梗死面积、灌流液中乳酸脱氢酶(LDH)含量、心肌组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)的活性。结果:各组在再灌注后各项指标均发生变化,在再灌注60 min时,模型组与正常组比较心率下降(125±9),(169±16)次/min(P<0.01);灌流量下降(3.48±0.43),(5.53±0.30)mL·min-1(P<0.01);梗死面积显著增加(19.57±2.34),(1.57±0.29)%(P<0.01);LDH漏出量增加(206.67±11.85),(120.96±13.56)U·L-1(P<0.01);SOD活性下降(99.14±8.24),(153.22±8.93)U·mg-1(P<0.01),MDA含量增加(6.17±0.73),(3.80±0.41)μmol·g-1(P<0.01)。与模型组比较,番茄红素各组心率和冠脉流量增加,梗死面积减少,LDH漏出量减少,心肌组织中MDA含量降低,SOD活性增加,各剂量组之间呈剂量依赖性。结论:番茄红素对豚鼠离体心脏缺血/再灌注损伤有保护作用,其作用机制可能与抗氧化应激相关。Objective： To explore the effects of lycopene on ischemia reperfusion injure in heart of isolated guinea pig. Method： Thirty isolated guinea pig hearts were divided into five groups：control group, model group, lycopene L group（2.5 mg·L^-1）, lycopene M group（5 mg·L^-1）, lycopene H group（10 m·gL^-1）. Heart rate（HR）, coronary flow（CF）, infarct size, lactate dehydrogenas （LDH） in effluent, malondiadehyde （MDA） and superoxide dismutase （SOD） in heart tissues were measured in different groups. Result： The indexes of each group are changed after reperfusion, when reperfusion 60 min, compared with the control group, model group showed that HR was decresed （1259）,（16916）stroke perminute-1, P〈0.01,CF decreased（3.480.43）,（5.530.30）mL·min^-1, P〈0.01; infarct size increased （19.572.34）%,（1.570.29）%, P〈0.01. the level of LDH increased（206.6711.85）,（120.9613.56）U·L^-1,P〈0.01, SOD activity decreased（99.148.24）,（153.228.93）U·mg^-1,P〈0.01; the level of MDA increased（6.170.73）,（3.800.41）μmolg-1,P〈0.01.Compared to model group, Lycopene group reduced infarct size and releases of LDH in effluent and MDA in heart tissues, increased HR, CF and the activity of SOD in heart tissues. Conclusion： The findings indicate that in the isolated guinea pig heart, lycopene can protect myocardium against ischemia/reperfusion injury and the mechanism could be related to lipid peroxidation in heart.

关 键 词：番茄红素 缺血再灌注损伤 离体心脏 脂质过氧化 

分 类 号：R285.5[医药卫生—中药学]