风信子鹿角珊瑚繁殖前后脂质与脂肪酸的变化  

Variation of lipid and fatty acid contents in the reef coral Acropora hyacinthus between prereproduction and postreproduction

作　　者：郭少华[1,2,3] 黄晖[1,2] 刘胜[1] 袁翔成 练健生[1] 雷新明[1] 陈标[1,2,3] 

机构地区：[1]中国科学院南海海洋研究所,广东广州510301 [2]中国科学院海南热带海洋生物实验站,海南三亚572000 [3]中国科学院大学,北京100049

出　　处：《热带海洋学报》2014年第1期74-80,共7页Journal of Tropical Oceanography

基　　金：中国科学院知识创新工程重要方向项目(kzcx2-yw-227);国家海洋局公益性项目(201105012-2;201005012-6);国家青年科学基金(41106107);LMEB201001

摘　　要：采用氯仿-甲醇混合液提取法及气相色谱-质谱联用法,首次对造礁石珊瑚(风信子鹿角珊瑚Acropora hyacinthus)繁殖前后脂质与脂肪酸的变化进行了研究。研究结果表明:1)繁殖过程对风信子鹿角珊瑚脂质含量、组织含量有较大影响。繁殖过后风信子鹿角珊瑚脂质含量减少约43.4%,组织含量减少约19.6%,而共生藻密度在繁殖过程中并无显著性变化;2)风信子鹿角珊瑚在繁殖前脂质含量大量累积,以顺利完成繁殖过程;3)首次表明二十碳五烯酸(C20:5n-3,eicosapntemacnioc acid,EPA)、二十二碳六烯酸(C22:6n-3,docosahexaenoic acid,DHA)是造礁石珊瑚繁殖过程中重要的脂肪酸,其含量在繁殖过程中具有显著变化。EPA含量繁殖前从15.7μg·gdw-1增加到22.16μg·gdw-1,增加41.1%,而繁殖后降为15.06μg·gdw-1,减少32.0%;DHA含量繁殖前从18.17μg·gdw-1增加到24.81μg·gdw-1,增加36.5%,而繁殖后降为16.31μg·gdw-1,减少34.3%。此外,多聚不饱和脂肪酸(poly-unsaturated fatty acids,PUFAs)含量在繁殖后也显著性减少;4)造礁石珊瑚繁殖时可能会适当增加摄食,以满足繁殖过程对脂肪酸和能量的需求。Abstract： Lipid and fatty acid contents were firstly monitored in the reef coral Acropora hyacinthus during itsreproductive cycle by using chloroform-methanol mixture extraction method and gas chromatography-massspectrometry. The results are summarized here. The lipid content and tissues of the reef coral A. hyacinthus fluctuatedmarkedly during the reproductive process, with the lipid content decreased drastically by approximately 43.4% and thetissues reduced by about 19.6% after reproduction. However, there was no significant difference in symbiotic algaedensity during the breeding process, implying that the eggs were probably without zooxanthellae. The reef coral A.hyacinthus accumulated lipid in the process of reproduction preparing. EPA （C20：5n-3, eicosapntemacnioc acid） andDHA （C22：6n-3, docosahexaenoic acid） played important roles in reproduction, since their contents changedsignificantly during this process. EPA and DHA increased by 41.3% and 36.5%, respectively, before breeding. However,their contents decreased by 32.0% and 34.3%, respectively, after breeding. Meanwhile, the content of poly-unsaturatedfatty acids （PUFAs） also reduced appreciably, suggesting that they were intimately bound up with the reproduction. Scleractinian corals likely increased feeding to meet the need of fatty acids and energy during the reproductive process.

关 键 词：繁殖 造礁石珊瑚 脂质 脂肪酸 

分 类 号：Q178.53[生物学—水生生物学]