缺氧对胃癌细胞BMPR-1A表达及其侵袭和迁移能力的影响  

The affect of hypoxia on the expression of BMPR- 1A and capability of invasion and migration in gastric cancer cells

作　　者：刘家铭[1] 王亚芳[1] 魏彦玲[1] 张宏博[1] 

机构地区：[1]第四军医大学西京医院消化科,陕西西安710032

出　　处：《现代肿瘤医学》2014年第3期502-506,共5页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81272349)

摘　　要：目的:检测胃癌细胞中BMPR-1A在常氧和缺氧条件下的表达;探讨两种不同条件下BMPR-1A的不同表达对胃癌细胞侵袭和迁移的影响。方法:常氧条件下(氧气浓度20%)常规培养胃癌细胞SGC7901和MKN28;缺氧条件下(氧气浓度1%)用二氧化碳/氧气/氮气三气培养箱培养胃癌细胞SGC7901和MKN2824小时。分别用蛋白质免疫印迹(Western blot)和实时定量聚合酶链反应(RT-PCR)的方法在蛋白水平和mRNA水平测定BMPR-1A的表达,以明确BMPR-1A在常氧与缺氧两种不同条件下的差异性表达;同时以Transwell实验检测在常氧和缺氧条件下由于BMPR-1A的差异性表达对胃癌细胞SGC7901和MKN28侵袭和迁移的影响。结果:缺氧条件下刺激胃癌细胞24h后,无论是在蛋白水平还是mRNA水平,BMPR-1A表达明显下降;Transwell实验结果也显示胃癌细胞接受缺氧刺激后其侵袭和迁移能力显著增强。结论:缺氧有增加胃癌细胞侵袭和迁移的能力,该作用机制可能与缺氧导致BMPR-1A的表达下降有关。Objective：To detect the expression of BMPR - 1A in the human gastric cancer cells SGC7901 and MKN28 under normoxia and hypoxia, and investigate the influence of the different expression of BMPR - 1A under normoxia and hypoxia on the invasion and migration in hmnan gastric cancer cells. Methods：The gastric cancer cells SGC7901 and MKN28 were cfultured routinely in normoxie condition （37℃ and 5% CO2）. in hypoxie condition （37℃ ,1% O2,5 % CO2, and 94% N2） , tile gastric cancer cells SGC7901 and MKN28 were cultured in CO2/O2/N2 three - gas incubator for 24h. The protein and mRNA levels of BMPR - 1A were determined by Western blot and real - time PCR （RT - PCR） assays . hffluenee of different expression of BMPR - 1A under normoxia and hypoxia on the invasion and migration of gastric cancer cells SGC7901 and MKN28 was detected using Transwell. Results：The re- suits of Western blot and quantitative real time PCR showed that BMPR - 1A was expressed down - regulated after the gastric cancer cells exposed to hypoxia for 24h;Transwell assay showed that the invasive and migratory ability were enhanced when the gastric cancer cells were exposed to hypoxia for 24h. Conclusion：Hypoxia could enhance the in- vasive and migratory ability of gastric cancer cells via down - regulating BMPR - 1A.

关 键 词：胃癌 缺氧 BMPR-1A 侵袭 迁移 

分 类 号：R730.23[医药卫生—肿瘤] R735.2[医药卫生—临床医学]