人参皂甙Rh2联合顺铂对食管癌细胞的杀伤作用  

The suppression effect of ginsenoside Rh2 combined with cisplatin on human esophageal cancer cell line Eca109

作　　者：李雪甫[1] 刘艳文[1] 王永实[1] 

机构地区：[1]淮阴卫生高等职业技术学校,江苏淮安223300

出　　处：《现代肿瘤医学》2014年第3期516-518,共3页Journal of Modern Oncology

基　　金：淮安市科技基金项目(编号:2009002-4)

摘　　要：目的:体外观察低剂量人参皂甙Rh2(ginsenoside Rh2,GS-Rh2)对抗肿瘤药顺铂(cisplatin,DDP)杀伤人食管癌细胞株Eca109的影响,并探讨其可能的作用机制。方法:人食管癌细胞Eca109经培养符合条件后,分别加用0.3μg/ml DDP(A组)、20μmol/ml GS-Rh2(B组)、0.3μg/ml DDP+20μmol/ml GS-Rh2(C组)、对照组(D组)处理48小时。流式细胞仪检测各组细胞凋亡率和死亡率。高效液相色谱检测各组细胞内DDP的药物浓度。蛋白质印迹法检测各组细胞中p53表达。结果:细胞凋亡率和死亡率C组显著高于A、B两组(P<0.05);C组细胞内DDP的药物浓度显著高于A组细胞(P<0.05);C组细胞p53表达水平低于A组(P<0.05)。结论:体外低剂量GS-Rh2能增强DDP对人食管癌细胞Eca109的杀伤作用,其作用机制可能与降低细胞内p53表达有关。Objective：To observe the effect of cisplatin on Eea109 cells by treatment with low dose of ginsenoside Rh2,and explore the preliminary mechanisms. Methods：The apoptosis was assayed by flow cytometry. HPLC was used to determine DDP. The expression of p53 was detected by Western blot. Results：The cell apoptosis and mortality in group C was significantly higher than A, B groups （P 〈 0.05）. Drug concentration of DDP within cells in group C was significantly higher than A two groups （P 〈 0.05）. But the p53 expression of group C was lower than A group （P 〈 0.05）. Conclusion： Eca109 cells with GS - RH2 increased the killing effect of DDP,and the mechanism is related to inhibiting the expression of p53 glycoprotein inside the cells.

关 键 词：食管肿瘤 人参皂甙RH2 顺铂 P53 细胞凋亡 

分 类 号：R735.1[医药卫生—肿瘤]