衍生化气相色谱法测定冠心病患者血清中游离脂肪酸的方法学研究  

Methodological study of serum free fatty acids in patients with coronary disease based on derivation reaction combined with gas chromatography-mass spectrometry

作　　者：李强[1] 王世杰[1] 胡小英[2] 卫阳飞[3] 戚欢阳[3] 

机构地区：[1]兰州大学第一医院心脏中心,甘肃兰州730000 [2]兰州军区机关门诊部药局,甘肃兰州730000 [3]中科院兰州化学物理研究所,甘肃兰州730000

出　　处：《检验医学》2014年第3期283-287,共5页Laboratory Medicine

基　　金：甘肃省自然科学基金项目(1010RJZA123);兰州大学"中央高校基本科研业务费"自由探索项目-面上项目(lzujbky-2011-102)

摘　　要：目的建立同时测定冠心病患者血清中11种游离脂肪酸(FFA)含量的测定方法。方法采用N,O-双(三甲基硅烷基)三氟乙酰胺(BSTFA)对11种FFA进行硅烷化反应,并利用气相色谱质谱联用技术(GC/MS)同时测定冠心病患者血清冻干粉中11种FFA的含量。结果 11种FFA色谱峰完全分离,峰形良好,血清中杂质对测定不产生干扰。在测定浓度范围内线性关系良好,11种FFA的回收率均>75%,日内、日间精密度和重复性实验的相对标准偏差(RSD)均<10%。结论该方法具有操作简单、灵敏度高、准确性好、线性范围宽等优点,可应用于临床实际血清样品中FFA的分析检测。Objective To establish a method for determining the serum concentrations of 11 free fatty acids（FFA） in patients with coronary disease.Methods The serum concentrations of 11 FFA in patients with coronary disease were determined by gas chromatography-mass spectrometry （GC/MS ） combined with derivation reaction and N,O-bis （trimethylsilyl）trifluoroacetamide（BSTFA）as derivative reagent.Results Chromatographic peaks of 11 FFA were separated completely,and all peak shapes were good.The interference of serum impurities on the determination did not occur.The curves showed good linearity.The recoveries of 11 FFA were 〉75%,respectively.The relative standard deviations（RSD）of intra-day and inter-day were〈10%,respectively.Conclusions The method is sensitive,accurate, easy to operate and time-saving.It provides referential basis to the determination of serum FFA in coronary disease.

关 键 词：游离脂肪酸 衍生化 气相色谱质谱联用 冠心病 

分 类 号：R446.1[医药卫生—诊断学]