即刻早期反应基因对肿瘤坏死因子相关凋亡诱导配体诱导乳腺癌细胞HCC1937凋亡的影响  

Immediate early response gene X-1 promotes the apoptosis of the breast cancer cell line HCC1937 induced by tumor necrosis factor-related apoptosis inducing ligand

作　　者：孙言波 刘世海[2] 刘相萍[2] 梁晔[2] 周泉[2] 王海波[2] 

机构地区：[1]青岛大学,266003 [2]青岛大学医学院附属医院

出　　处：《中华实验外科杂志》2014年第5期941-943,F0003,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81372632）;山东省教育厅资助项目（J11LF05）

摘　　要：目的观察即刻早期反应基因（IER3）在肿瘤坏死因子相关凋亡诱导配体（TRAIL）诱导乳腺癌细胞HCC1937凋亡中的作用。方法采用实时定量聚合酶链反应（Real-timePCR）检测TRAIL和IER3干预48h前后TRAIL和IER3基因表达改变，分别于24、48、72、96、120h后采用噻唑蓝（MTT）法检测HCC1937细胞的增殖，10～14d后采用细胞克隆形成实验检测细胞克隆形成能力，24h后采用Hoechst33258染色检测细胞凋亡。结果与阴性对照组比较，TRAIL组和IER3＋TRAIL组的TRAIL相对表达量分别是42．69±3．88和46．36±3．55；IER3组和IER3＋TRAIL组的IER相对表达量分别是5．08±1．40和9．66±2．25。TRAIL、IER3及TRAIL＋IER3组在120h时的抑制率（％）分别为（50．95±7．45）、（25．39±4．49）及（72．01±2．95）。IER3＋TRAIL组的细胞克隆形成能力明显降低，而细胞凋亡率明显增高。结论IER3能增强TRAIL诱导乳腺癌细胞HCC1937的凋亡，两者联合具有协同诱导细胞凋亡的作用。Objective To observe the effect of immediate early response gene X-1 （IER3） on the apoptosis of the breast cancer cell line HCC1937 induced by tumor necrosis factor-related apoptosis inducing ligand （TRAIL）. Methods The expression of gene IRAIL and IER3 was detected before and after treatment by real-time quantitative polymerase chain reaction （Real-time PCR） at 48 b, The proliferation of breast cancer cells HCC1937 was mearsured by using Methylthiazol tetrazolium （MTF） assay at 24, 48, 72, 96, and 120 h, The clone formation ability was mearsuerd by clone formation assay after 10-14 d, breast cancer cells HCC1937 were examined by Hoechst 33258 stain to detect the apoptpsis after 24 h. Results Compared with the negative control group, the relative expression of TRAIL in the TRAIL and the IER3 ＋ TRAIL groups were 42. 69 ± 3.88 and 46. 36 ± 3.55 times respectively. And the relative expression of the IER3 in the IER3 and the IER3 ＋ TRAIL groups were 5.08 ± 1.40 and 9. 66 ± 2. 25 times respec- tively. The inhibition rate （ % ） of cells in TRAIL, IER3 and TRAIL ＋ IER3 groups were （ 50. 95 ＋ 7.45）, （25.39 ±4.49） and （72.01 ±2. 95） respectively. The cell clone formation ability in IER3 ＋ TRAIL group was obviously reduced. Compared with the negative control group, the apoptosis rate of cells in IER3 ＋ TRAIL group was also increased significantly. Conclusion IER3 could promote the apoptosis of the breast cancer cell line HCC1937 induced by TRAIL.

关 键 词：即刻早期反应基因 肿瘤坏死因子相关凋亡诱导配体 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]