鱼藤素对结肠癌Lovo细胞上皮-间充质转化及侵袭的影响  

Effects of duguelin on epithelial-mesenchymal transition and invasion of rectal cancer lovo cells

作　　者：姜建武 李小兰[1] 冷彦[1] 陶德定[1] 胡俊波[1] 龚建平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院分子医学中心,武汉430030

出　　处：《中华实验外科杂志》2014年第5期1018-1020,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81101943、81171927、81372324、81201638）

摘　　要：目的观察探讨鱼藤素对结肠癌Lovo细胞上皮-间充质转化（EMT）以及侵袭能力的影响。方法分别用Westernblot法和实时定量聚合酶链反应（Real—timePCR）法检测鱼藤素对E-钙黏蛋白（E—cadherin）、Twist、Snail、波形蛋白（Vimentin）在蛋白和mRNA水平表达的影响；划痕实验和Transwell侵袭实验检测鱼藤素对Lovo细胞侵袭能力的影响。结果1μmol／L鱼藤素作用于结肠癌Lovo细胞0、24、48h后，蛋白水平Twist的相对表达量分别为1．40±0．07、0．41±0．07和0．30±0．03；Snail的相对相对表达量分别为1．04±0．11、0．16±0．04和0．23±0．02；Vimentin的相对表达量分别为4．31±0．30、1．30±0．27和1．21±0．21；E—cadherin的相对表达量分别为2．62±0．33、3．96±0．27和6．89±0．16。mRNA水平Twist的相对表达量分别为0．63±0．09和0．50±0．06；Snail的相对表达量分别为0．46±0．06和0．51±0．19；Vimentin的相对表达量分别为0．76±0．05和0．57±0．04；E—cadherin的相对表达量分别为1．88±0．27和2．29±0．60。1μmol／L鱼藤素作用于结肠癌Lovo细胞24、48h后，相对迁移率分别为（20．23±5．16）％和（27．94±6．66）％，而无鱼藤素的对照组24、48h后相对迁移率则分别为（45．65±4．41）％和（66．14±3．31）％（P〈0．01）。Transwell侵袭实验证实鱼藤素组每个视野穿透细胞数为（130．3±16．19）个，而对照组为（459．0±14．19）个（P〈0．01）。结论鱼藤素能够有效抑制结肠癌Lovo细胞的EMT及侵袭。Objective To investigate the effects of deguelin on epithelial-mesenchymal transition and invasion of rectal cancer lovo ceils. Methods Western blotting and real-time quantitative polymerase chain reaction （Real-time PCR） were used to examine the change of E-cadherin, Twist, Snail and Vimentin at protein and mRNA levels. Invasive ability was assessed through wound scrach assay and Transwell assay. Results After treated with duguelin 1 μmol/L for 0,24 and 48 h in lovo cells, the relative density ratio of Twist were （ 1.40 ± 0. 07 ） , （ 0. 41 ± 0. 07 ） and （ 0. 30 ± 0. 03 ） respectively ; of Snail were （ 1.04 ± 0. 11 ） , （ 0. 16 ± 0. 04 ） and （ 0. 23 ± 0. 02 ） respectively ; of Vimentin were （ 4. 31 ± 0. 30 ）, （ 1.3 ± 0. 27 ） and （ 1.21 ± 0. 21 ） respectively ; of E-eadherin were （ 2. 62 ± 0. 33 ）, （ 3.96 ± 0. 27 ） and （ 6. 89 ± 0. 16 ） respectively in protein level. In mRNA level, after treated with duguelin 1 p, mol/L for 24 and 48 h in lovo cells, the relative expression of Twist were （0. 63 ± 0.09 ） and （ 0. 50 ± 0. 06 ） respectively; of Snail were （ 0. 46 ± 0. 06 ） and （ 0. 51 ± 0. 19 ） respectively; of Vimentin were （ 0. 76 ± 0. 05 ） and （ 0. 57 ± 0. 04 ） respectively ; of E-cadherin were （ 1.88 ± 0. 27） and （ 2. 29 ± 0. 60） respectively. Scrach assay showed that the relative migration rate of Lovo cells treated with duguelin 1 p,mol/L for 24,48hours were （20. 23 ± 5.16） % and（27.94 ± 6.66） % respectively, compared with（45.65 ± 4.41 ） % and（66. 14 ± 3.31 ） % in control group（P 〈 0. 01 ）. Comparing to （459.0 ± 14. 19） cells invaded in control group, deguelin decreased invaded cells to （ 130. 3 ± 16. 19, P 〈 0. 01 ）. Conclusion The epithelial-mesenchymal transition and invasion of lovo cells can be inhibited strongly by duguelin.

关 键 词：鱼藤素 结肠癌 上皮-间充质转化 侵袭 

分 类 号：R735.35[医药卫生—肿瘤]