人X连锁甲基化CpG结合蛋白2在帕金森病细胞模型中的表达及意义  

Distribution and role of methyl-CpG-binding protein 2 in cell model of parkinson＇ s disease

作　　者：谢腾[1] 罗勇[1] 陈治军[1] 刘平非[1] 张捷[2] 袁先厚[2] 

机构地区：[1]湖北省荆门市第一人民医院神经外科,448000 [2]武汉大学中南医院神经外科

出　　处：《中华实验外科杂志》2014年第5期1033-1035,共3页Chinese Journal of Experimental Surgery

基　　金：湖北省荆门市科技计划资助项目（2013S01）

摘　　要：目的观察甲基化CpG结合蛋白2（MeCP2）在帕金森病细胞模型中的表达。方法采用免疫荧光技术、Westernblot检测MeCP2、酪氨酸羟化酶（TH）蛋白在帕金森病细胞模型中表达的变化。结果在正常SH．SY5Y细胞中有MeCP2、TH表达，而在帕金森病细胞模型中，免疫荧光检测结果显示MeCP2、TH的表达降低，Westernblot结果进一步证实MeCP2、TH的表达下降，MeCP2／β-肌动蛋白（β—actin）由（86．97±4．62）％下降至（22．19±2．85）％，TH／β—actin南（49．92±2．35）％下降至（9．02±0．84）％。结论MeCP2在帕金森病的发病过程中起重要作用，MeCP2可能参与了帕金森病的发病过程。Objective To investigate the regularity of methyl-CpG-binding protein 2 （MeCP2） expression in cell model of Parkinson＇ s disease. Methods Detected the expression of MeCP2 and tyrosine hydroxylase （TH） in cell model of Parkinson＇ s disease by immunofluorescence and Western blotting. Results We found the MeCP2/TH protein expression in SH-SYSY cell by immunofluorescence and Western blotting, and also the MeCP2 protein and TH protein expression reduced in the cell model of Parkinson＇ s disease. The expression of MeCP2 protein and TH protein reduced were further confirmed by Western blotting analysis, the ratio of MeCP2/β-actin from （86. 97 ± 4. 62） % decreased to （22. 19 ± 2. 85 ） %, and the ratio of TH/13-actin from （49.92 ± 2.35 ） % decreased to （9. 02 ± 0. 84） %. Conclusion MeCP2 plays an important role in the pathogenesis of Parkinson＇ s disease, MeCP2 may be involved in the pathogenesis of Parkinson＇ s disease.

关 键 词：帕金森病 甲基化CpG结合蛋白2 酪氨酸羟化酶 SH—SY5Y细胞 6-羟基 多巴胺 

分 类 号：R742.5[医药卫生—神经病学与精神病学]