恩度联合放疗对肿瘤组织血流灌注和血管生成的影响  

Effect of Endostar combined with radiotherapy on tumor tissue perfusion and angiogenesis of Lewis lung cancer

作　　者：戈伟[1] 明平坡[1] 陈文卫[2] 徐慧琳[1] 李长虎[1] 郑永法[1] 徐细明[1] 陶泽璋[3] 李桂兰[1] 

机构地区：[1]武汉大学人民医院肿瘤中心,430060 [2]武汉大学人民医院超声影像科,430060 [3]武汉大学人民医院耳鼻咽喉头颈外科,430060

出　　处：《中华实验外科杂志》2014年第5期1051-1053,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81272500、30970860）

摘　　要：目的观察恩度联合放疗对Lewis肺癌小鼠肿瘤组织血流灌注和血管生成的影响。方法从治疗当天开始，隔日测量肿瘤体积，绘制肿瘤生长曲线，同时采用局部瘤体直接团注声诺维的方法行超声造影检查，使用SonoLiver1．0软件分析造影过程中获取的时间一强度曲线（TIC），动态血管模式曲线（DVP）及TIC各项参数的变化。结果荷瘤小鼠肿瘤体积随时间不断增长，而恩度联合放疗组与其余3组比较，在第6天后生长速度明显变慢，差异有统计学意义（P〈0．05）。TIC参数始增时间（s）、达峰时间（s）、上升支斜率（dB／t）、感兴趣区增强强度（dB）、半降时间（s）在不同的测量时间点之间差异有统计学意义（P〈0．05）。峰值强度（IMAX）、曲线下面积（AUC）随着肿瘤生长时间呈上升趋势，但在后期由于肿瘤内出现明显坏死，出现下降。结论恩度联合放疗可抑制Lewis肺癌小鼠肿瘤的生长，并影响小鼠肿瘤新生血管生成，使其分布规则，改善肿瘤组织血流灌注，超声造影检查可观察荷瘤小鼠肿瘤内血流灌注和坏死区域的变化，显像效果良好。Objective To observe the effect of Endostar combined with radiotherapy on tumor tissue perfusion and angiogenesis of Lewis lung cancer. Methods The tumor size was measured and tumor growth curve drawn every other day, and contrast enhanced ultrasound wad used by directly injecting agent into tumor tissue. The software （ SonoLiver 1.0） was used to obtain the time intensity curve of contrast analysis process （TIC） and dynamic vascular pattern curve （DVP）. Results Tumor volume was increased with time, and the increasing rate in ES ＋ RT group was obviously slower since D6 than other three groups （ P 〈 0. 05 ）. Differences in enhancement beginning time （ s）, time to peak （ s）, ascending slope （ dB/t）, enhanced strength of region of interest （ dB）, half time of descending （s） among time points showed statistical significance （ P 〈 0. 05 ）. The peak intensity （IMAX） and area under the curve （ AUC ） showed an increased trend with the time of tumor growth, but in the late stage declined changes occurred due to obvious necrosis in the tumor. Conclusion Endostar combined with radiotherapy could significantly inhibit the growth of tumor in mice with Lewis lung cancer and can influence the expression of tumor angiogenesis, improve the perfusion of tumor in mice. Contrast enhanced ultrasound can observe the change of tumor blood perfusion and necrosis area of the tumor, and exerts good reproducibility in quantifying the perfusion.

关 键 词：超声造影 肺癌 血流灌注 恩度 放射治疗 

分 类 号：R730.55[医药卫生—肿瘤] R734.2[医药卫生—临床医学]