谷氨酸棒杆菌生产琥珀酸的代谢工程研究进展  

Progress in metabolic engineering of Corynebacterium glutamicum for succinate production

作　　者：夏慧华[1] 尹国民 朱年青[1] 陈涛[1] 

机构地区：[1]天津大学化工学院教育部系统生物工程重点实验室,天津300072 [2]烟台只楚药业有限公司,山东烟台264002

出　　处：《微生物学通报》2014年第5期943-949,共7页Microbiology China

基　　金：国家973计划项目(No.2011CBA00804;2012CB725203);天津市自然科学基金项目(No.12JCYBJC12900);教育部博士点基金项目(No.20100032120014)

摘　　要：琥珀酸是一种具有重要应用价值的四碳平台化合物。微生物法发酵生产琥珀酸以其社会、环境和经济优势展现出良好的发展前景。谷氨酸棒杆菌被广泛应用于氨基酸、核苷酸等高附加值化学品的工业化生产,在厌氧条件下细胞处于生长停滞状态,但仍能高效利用碳源合成有机酸,通过代谢工程改造的谷氨酸棒杆菌有望成为理想的琥珀酸生产菌株。结合近年来谷氨酸棒杆菌生产琥珀酸取得的最新成果,本文综述了构建高产琥珀酸工程菌株的代谢工程策略、底物的扩展利用,并展望了将来的研究方向。Succinic acid is an important four-carbon platform compound that is widely applied in the pharmaceutical, agricultural and food industry. Biological processes for succinic acid production show a good prospect for its social, environmental and economic benefits. Corynebacterium glutamicum is broadly used for industrial production of value-added chemicals such as amino acid and nucleotide. Under anaerobic conditions, C. glutamicum cell growth is arrested, but the cells retain the capability to metabolize sugars to various organic acids efficiently. It is thus becoming a desired succinate-production strain by means of metabolic engineering. Combining with the latest achievements of succinic acid production with C. glutamicum, this mini-review summarized the metabolic engineering strategies of constructing an efficient succinate-production strain, the expansions of substrate utilization, and the prospects of future research.

关 键 词：谷氨酸棒杆菌 琥珀酸 代谢工程 底物利用 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]