多烯类抗生素合成基因簇中ABC转运蛋白研究进展  

Research progress of ATP-binding cassette transporters in polyene antibiotic biosynthesis gene cluster

作　　者：李涵[1,2] 刘剑波[1,2] 汪谭俊 江辉[1,2] 张仁炳[3] 管文军[1,2] 

机构地区：[1]浙江大学生命科学学院,浙江杭州310058 [2]浙江省微生物代谢与工程重点实验室,浙江杭州310058 [3]浙江大学校医院,浙江杭州310058

出　　处：《微生物学通报》2014年第5期950-958,共9页Microbiology China

基　　金：国家自然科学基金项目(No.31170032);国家863计划项目(No.2012AA022107)

摘　　要：ABC转运蛋白家族是一个广泛存在于不同生物细胞中且功能保守的膜蛋白亚家族;它们是一类单向底物转运泵,通常以主动转运方式完成多种分子的跨膜转运。随着抗生素合成基因簇相关研究的开展,越来越多的簇内ABC转运蛋白被鉴定出来,对其生物学功能的研究正逐渐成为热点。多烯类抗生素作为一类重要的抗真菌药物,能够有效避免真菌产生耐药性,具有非常重要的临床价值。本文以多烯类抗生素合成基因簇为对象,综述了在其中所发现的ABC转运蛋白的研究进展,综合分析了其结构特性与功能间的关系,并对研究应用进行了展望。ATP-binding cassette （ABC） transporters are members of a protein superfamily that is one of the largest and most ancient families with great variety of functions. They are involved in trans- porting different substrates across the plasma membrane. As more ABC transporters were identified from the gene clusters for antibiotic biosynthesis, recent studies have begun to focus on their bio- logical functions. The polyene antibiotics represent a class of biologically active fungal metabolites synthetized by the genus Streptomyces. As drug resistance to polyene remains rare, polyene antibiot- ics have been the cornerstone of therapy for critically ill patients with invasive fungal infections. We reviewed the latest research progresses on ABC transporters of polyene antibiotic biosynthesis gene clusters, including their structure characteristics and biological functions. The potential relationship between the secondary structure and their function is also discussed.

关 键 词：ABC转运蛋白 基因簇 多烯类抗生素 链霉菌 

分 类 号：R341[医药卫生—基础医学]